Dataset Information

Expression data from the transplanted kidney and the native kidney of NEP25 mice with/without the treatment of LMB2

ABSTRACT: A previous study revealed the appropriate amount of LMB2 (1.75 ng/g body wt dissolved in 0.1 ml of PBS containing 0.1% bovine serum albumin [BSA]) needed to induce reproducible a focal segmental glomerulosclerosis (FSGS) lesions in NEP25 mice. We performed kidney transplantation between NEP25 mice and their wild-type littermates (WTm) which have the same genetic background (C57BL/6N). In this study, the transcriptomic profiles of transplanted and native kidneys with/without LMB2 in nephrotic model mice were investigated using the next-generation RNA sequencing (RNA-seq). Reads were mapped on mm10 mouse reference genome and quantified using CLC Genomics Workbench version 10.1.1 (QIAGEN).

ORGANISM(S): Mus musculus

PROVIDER: GSE125840 | GEO | 2025/06/04

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Recurrence of focal segmental glomerulosclerosis (rFSGS) after kidney transplantation is a cause of early and accelerated graft loss. Immuneadsorption can alleviate renal dysfunction and suggests that circulating antibodies (Ab) are likely implicated in disease pathogenesis. To evaluate pathogenic Ab in rFSGS, we processed 141 unique serum samples from patients with and without primary rFSGS (n=64) and 34 non-FSGS control, transplanted at five (US and EU) hospitals. 9000 antigens were screened in pre-transplant sera by protein arrays and 10 Ab targeting glomerular antigens were selected for ELISA validation. A panel of 7 Ab (CD40, PTPRO, CGB-5, FAS, P2RY11, SNRPB2 and APOL2) could predict post-transplant FSGS recurrence with 92% accuracy. Pre-transplant elevation of anti-CD40 Ab levels alone had a substantial impact (78% accuracy) on the identification of rFSGS risk after transplantation. Epitope mapping of CD40 with customized peptide arrays and rFSGS sera demonstrated altered immunogenicity of the extracellular CD40 domain in rFSGS. Immunohistochemistry of CD40 demonstrated a differential expression of these antigens in FSGS compared to non-FSGS. Anti-CD40 Ab purified from rFSGS patients were uniquely pathogenic in human podocyte cultures; injection of these Ab resulted in heightened proteinuria, independently and in combination with suPAR in a rodent model, abrogated by injection of monoclonal Ab to CD40. In conclusion, a panel of 7 Ab can identify primary FSGS patients at high risk of recurrence prior to transplantation, allowing for customized therapies and improved patient selection for transplant. Intra-renal CD40 is an important axis of disease pathogenesis, and human trials of anti-CD40 therapies are warranted to evaluate their efficacy in preventing rFSGS and improving graft survival. The purpose of the study was to identify potential auto-Abs associated with rFSGS. We used a discovery set of pre-transplant sera from 20 unique patients with biopsy confirmed diagnosis of primary FSGS as their cause of ESRD, of which 10 had progressed to rFSGS within the first post-transplant year and 10 did not have recurrence of proteinuria or histological disease after transplantation (nrFSGS).

Dataset Information

Expression data from the transplanted kidney and the native kidney of NEP25 mice with/without the treatment of LMB2

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets