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The effect of prohibitin overexpression in Caco2-BBE intestinal epithelial cells

ABSTRACT: To determine the effect of prohibitin overexpression on global gene expression in Caco2-BBE intestinal epithelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE12605 | GEO | 2008/09/05

SECONDARY ACCESSION(S): PRJNA112865

REPOSITORIES: GEO

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The effect of prohibitin overexpression in Caco2-BBE intestinal epithelial cells

Project description:To determine the effect of prohibitin overexpression on global gene expression in Caco2-BBE intestinal epithelial cells. 4 individual wells of Caco2-BBE cells, passage 41, were transfected with either empty vector (pcDNA4) or prohibitin/pcDNA4 for 72 hours. Total RNA isolated from 4 wells of cells/per treatment were pooled together for labeling and hybridization purposes.

2010-06-19 | E-GEOD-12605 | biostudies-arrayexpress

Homo sapiens

Project description:The effect of prohibitin overexpression in Caco2-BBE intestinal epithelial cells

| PRJNA112865 | ENA

Transcription profiling of mouse with intestinal epithelial cell-specific CD98 overexpression

Project description:Transcription profiling of mouse with intestinal epithelial cell-specific CD98 overexpression. In the result genes with fold changes of at least 1.5-fold relative to WT mice were selected: see E-MTAB-486.additional.zip.

2011-01-12 | E-MTAB-486 | biostudies-arrayexpress

Proteomics of campylobacter exposed to host Caco-2 and INT407 intestinal cells

Project description:Comparison of Campylobacter proteome in MH media with and without deoxycholic acid, in presence of FBS or after being exposed to INT 407 and Caco2 intestinal epithelial cells.

2019-05-03 | PXD009817 | Pride

The effect of CHRDL2 overexpression on gene expression in CACO2 CRC cells

Project description:Chordin-like-2 (CHRDL2) is a secreted BMP antagonist, with overexpression and genomic variants associated with colorectal cancer (CRC) risk. BMP signalling in the normal intestinal epithelium operates in opposition to the WNT signalling pathway, which maintains stem-cells and self-renewal. Elevated WNT signalling leads to expansion of the stem cell compartment and hyperproliferation, defining characteristics of CRC. Here, we explored the impact of CHRDL2 overexpression on CRC cells to investigate whether CHRDL2's inhibition of BMP signalling intensified WNT signalling, and enhanced the cancer stem-cell phenotype. RNA-seq analysis revealed that CHRDL2 increased the expression of stem cell markers and well-established cancer-associated pathways. We suggest that CHRDL2 overexpression can augment the stem-cell potential of CRC and normal intestinal cells.

2024-01-22 | GSE253554 | GEO

Proteomics analysis of inflammation mouse intestine model and Caco2 cells

Project description:Proteomics analysis of a mouse model for intestinal inflammation (IBD) and Caco2 cells used as inflammation model.

2020-11-09 | PXD020245 | Pride

Differential gene expression between immature and mature enterocyte like-Caco2 cells

Project description:Human Caco2 intestinal cells represent an in vitro model of human enterocytes. These cells are able to acquire a phenotype of mature polarized enterocytes when they are maintained in transwell culture system for 2-3 weeks.The goal of this study was to analyze the differences in mRNA expression profiles between Caco2 cells at a early stage of their differentiation (day 8) and at a more advanced stage of their differenciation (day 16) into absorptive enterocytes. A 3' Digital Gene expression (DGE) RNA sequencing was performed to determine mRNA expression profiles.

2021-09-22 | GSE162633 | GEO

Effect of O-GlcNAc transferase deficiency on intestinal epithelial cells in mice

Project description:O-GlcNAcylation is the modification of serine and threonine residues with beta-N-acetylglucosamine (O-GlcNAc) on intracellular proteins. To investigate the role of protein O-GlcNAcylation on intestinal homeostasis, we generated intestinal epithelial cell (IEC)-specific O-GlcNAc transferase (OGT) knockout in mice. The KO mice developed spontanous intestinal inflammation. To determine the underlying molecular mechanisms, we performed RNA sequencing of ileum and colon epithelial cells of wildtype and IEC-OGT KO mice.

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Homeostatic intestinal epithelial gene expression

Project description:Intestinal epithelial cells of 28-day-old female mice were isolated according to a recently published protocol (Lotz et al., J Exp. Med. 2006). Isolated intestinal epithelial cells of four individual animals (four biological replicates) were examined. Single color array.

2014-08-23 | E-GEOD-51269 | biostudies-arrayexpress

RNA-seq data of intestinal epithelial cells

Project description:We report that adhesion of microbes to intestinal epithelial cells is a critical cue for Th17 induction. SFB colonized in the intestine of mice can adhere to mouse small intestinal epithelial cells and induce intestinal Th17 cells. However, SFB colonized in rats cannot adhere to mouse intestinal epithelial cells and induce Th17 cells. Likewise, Citrobacter rodentium (WT) can adhere to mouse colonic epithelial cells and induce Th17 cells, but non-adherent mutant of C. rodentium (Δeae) cannot induce Th17 cells. To examine the influence of adherent bacteria on intestinal epithelial cells, we performed RNA seq. Germ free mice were orally inoculated with M-SFB or R-SFB and total RNA was isolated from small intestinal epithelial cells 1 week after inoculation. Alternatively, germ free mice were orally inoculated with C. rodentium WT or eae mutant and total RNA was isolated from colonic epithelial cells 5 days after inoculation. The gene expression of small intestinal epithelial cells isolated from small intestine of germ free mice (2 mice), mice monocolonized with M-SFB (2 mice) or R-SFB (3 mice), and colon of germ free mice (3 mice), mice monocolonized C. rodentium WT (3 mice) or eae mutant (3 mice).

2015-09-24 | E-GEOD-71734 | biostudies-arrayexpress

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