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Comparative transcriptomic analyssi of mouse embryonic tissues and mESCs-derived limb bud-organoid tissues

ABSTRACT: We established the differentiation method of a limb bud organoid from mouse embryonic stem cells (mESCs) using SFEBq. mESCs-derived limb bud organoid selectively differentiate into forelimb or hindlimb by adjusting the retinoic acids activity. To evaluate a correlation of gene expression between limb bud organoid and embryonic tissues (limb bud, branchial arch, cardiac, and tail bud), we performed comparative transcriptome analysis using RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE126387 | GEO | 2019/05/01

REPOSITORIES: GEO

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Comparative transcriptomic analyssi of mouse embryonic tissues and mESCs-derived limb bud-organoid tissues

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