Budding yeast centromeric DNA and A+T rich bacterial DNA can function as centromeres in the fission yeast Schizosaccharomyces pombe 

ABSTRACT: We established a two-step approach to centromere-replacement (Figure 1A in outline, and Supplementary data in detail). FiC31 integrase was used to place a candidate sequence or an empty vector adjacent to the native centromere of chromosome 2 of CBS2777, and Bxb1 integrase was subsequently used to delete the native centromere. 

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE126511 | GEO | 2019/07/10

REPOSITORIES: GEO