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Transcriptomic analysis of iPSC and ESC challenged with atmospheric or physiological oxygen

ABSTRACT: Modulation of metabolism by 5% (physiological) oxygen during reprogramming has been shown to improve reprogramming efficiency, yet it is not known how it may affect the transcriptome and physiology of iPSC. RNA-seq analysis highlighted that iPSC derived under 20% oxygen displayed transcriptomic instability. Consequently, inappropriate modulation of metabolism by atmospheric oxygen during reprogramming significantly impacts the resultant iPSC transcriptional landscape.

ORGANISM(S): Homo sapiens

PROVIDER: GSE126785 | GEO | 2019/02/21

REPOSITORIES: GEO

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