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Laser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex

ABSTRACT: We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus2.0 GeneChips. In the first instance, we used the dataset to compare the neuronal and endothelial data, in order to demonstrate that the predicted differences between cell types could be detected using this methodology. Keywords: cell type comparison, laser capture microdissection

ORGANISM(S): Homo sapiens

PROVIDER: GSE12679 | GEO | 2008/12/18

SECONDARY ACCESSION(S): PRJNA112675

REPOSITORIES: GEO

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Laser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex

Project description:We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus2.0 GeneChips. In the first instance, we used the dataset to compare the neuronal and endothelial data, in order to demonstrate that the predicted differences between cell types could be detected using this methodology. Keywords: cell type comparison, laser capture microdissection The dataset consists of endothelial cell samples from 18 individuals and neuronal samples from an overlapping population of 18 individuals.

2008-12-18 | E-GEOD-12679 | biostudies-arrayexpress

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Project description:We used laser capture microdissection (LCM) to capture soybean seed compartments and profiled the transcriptomes of several compartments using next-generation sequencing. We profiled the transcriptomes of the embryo-proper and the suspensor region from globular stage embryos and the seed coat parenchyma layer of early maturation stage seeds using the Illumina GAIIx system.

2011-05-10 | GSE29162 | GEO

Gene expression of invasive lobular carcinoma tumor epithelium and tumor stroma isolated by laser-capture microdissection

Project description:We used laser-capture microdissection (LCM) to separate tumor epithelium (TE) from stroma (TS) in 23 ILC samples. Gene expression analysis was used to identify genes that are enriched in the stroma of ILC, but not IDC or normal breast.

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Identification of Inner Mucus-Associated Bacteria by Laser Capture Microdissection

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RNA-seq of human and mouse brain microvessels isolated by laser capture microdissection and matched whole brain samples

Project description:Brain microvessels form the blood-brain barrier, and are dysfunctional in several neurological disorders. Brain microvessels are formed by brain microvascular endothelial cells (BMECs) and pericytes, and the molecular constituents of these cell types remain incompletely characterized, especially in humans. To improve molecular knowledge of these cell types and identify species differences in gene expression, we performed RNA-sequencing on brain microvessels isolated from human and mouse tissue samples using laser capture microdissection. We also performed RNA-sequencing of matched whole brain samples to identify genes with microvessel-enriched expression.

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Analysis of conditional loss of Geminin in neural tube and paraxial tissues at E10.5 by laser capture microdissection

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Project description:We used laser capture microdissection (LCM) to capture globular stage common bean embryo proper and suspensor, and profiled the transcriptome of these two embryo regions using next-generation sequencing. Our long-term goal is to understand the region-specific differentiation processes that occur during early embryo development and how genes are activated specifically in the suspensor and embryo proper.

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