Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from mouse precision cut lung slices (PCLS) after human rhinovirus (RV) infection and after treatment with bronchobini (BRO).

ABSTRACT: Transcriptome analysis revealed a robust and multifactorial mode of action of BRO. BRO suppressed RV induced responses, e.g. upregulation of the antiviral interferon (IFN) signaling pathway, while stimulating this pathway under baseline conditions. Suppression of RV-induced cytokine release and downregulation of IFN signaling pathway confirmed that BRO exerts a beneficial anti-inflammatory effect during viral infection.

ORGANISM(S): Mus musculus

PROVIDER: GSE126832 | GEO | 2019/06/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Rupintrivir reduces RV-induced T<sub>H</sub>-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo.

Project description: Not available

| S-EPMC6805592 | biostudies-literature

Expression data from mouse precision cut lung slices (PCLS) after human rhinovirus (RV) infection and after treatment with bronchobini (BRO).

Project description:Expression data from mouse precision cut lung slices (PCLS) after human rhinovirus (RV) infection and after treatment with bronchobini (BRO).

| PRJNA523417 | ENA

miRNA profiling in human precision-cut lung slices (PCLS)

Project description:Transcriptome analysis revealed miRNA profiles from PCLS of tumor-free tissue comparable to normal lung tissue Suitability of human PCLS as a model for lung-specific miRNA investigations by RTqPCR and microarray analyses

2021-06-22 | GSE167705 | GEO

Identification of candidate rhinovirus C (RV-C) receptors by gene expression analysis

2014-11-01 | GSE61396 | GEO

A modified protocol for successful miRNA profiling in human precision-cut lung slices (PCLS).

Project description: Not available

| S-EPMC8252208 | biostudies-literature

Identification of candidate rhinovirus C (RV-C) receptors by gene expression analysis

Project description:Members of rhinovirus C (RV-C) species are more likely to cause wheezing illnesses and asthma exacerbations compared to other rhinoviruses. The cellular receptor for these viruses was heretofore unknown. We measured gene expression (Human Gene 1.0 ST Array, Affymetrix) in two series of experiments involving cells that were either susceptible or not susceptible to RV-C infection. In one experimental series, susceptible cells included whole sinus mucosal tissue specimens (n = 5), epithelial cell suspension from sinus tissue, and nasal epithelium obtained via brushing, while non-susceptible cells included monolayers of primary undifferentiated epithelial cells and transformed cell lines (n = 5). In a second experimental series, we compared three pairs of undifferentiated and fully differentiated (ALI) sinus epithelial cell cultures. We identified a total of 12 genes upregulated in RV-C susceptible cells (represented by 14 probe sets) encoding proteins localized to plasma membrane, and/or with predicted or functionally demonstrated receptor activity, including members of the Human MHC class II, stomatin, guanine nucleotide-binding, type I cytokine and atypical chemokine receptor and cadherin protein families. Sinus and bronchial epithelial tissue samples were obtained from residual surgical specimens. Primary airway epithelial cells were cultured submerged (undifferentiated monolayers) or at air-liquid interface (fully-differentiated). Established cell lines (HeLa, NCI-H358 and WisL) were cultured submerged. Epithelial tissue samples were placed in RNAlater solution (Life Technologies) to stabilize and protect cellular RNA; cultured cells were lysed directly and total RNAs were isolated using TRIzol reagent (Life Technologies). Gene expression was analyzed using the Human Genome 1.0 ST GeneChip arrays (Affymetrix, Santa Clara, CA).

2014-11-01 | E-GEOD-61396 | biostudies-arrayexpress

Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models.

Project description: Not available

| S-EPMC6631151 | biostudies-literature

Contrasting gene expression patterns associated with high and low rhinovirus stimulated IFN-α production

Project description:Introduction: Viral respiratory infections cause considerable morbidity and economic loss. While rhinoviruses (RV) typically cause little more than the common cold, they can produce severe infections and disease exacerbations in susceptible individuals, including those with chronic lung diseases. Variations in the regulation of key antiviral cytokines, particularly type I interferon (IFN-α and IFN-b), may contribute to RV susceptibility, but are not well understood. Method: Whole genome transcription patterns were assessed in samples from 74 individuals demonstrating high or low IFN-α production following RV stimulation in vitro. Results: In unstimulated samples (baseline) the high IFN-α producer group showed higher expression of genes associated with plasmacytoid dendritic cells, the complement system, B-cell chemokines, immune regulation, macrophages, and vitamin D activation and lower expression of genes linked to haemoglobin and haem biosynthesis, wound healing, stress response and pro-inflammatory genes than the low IFN-α producer group. After RV stimulation, the high IFN-α producer group showed more intense upregulation of diverse immune function genes including IFNs and greater downregulation of genes linked to structural and extra-cellular matrix functions than the low IFN-α producer group. Patterns of antibacterial gene expression varied between groups. Healthy people and those with asthma generally showed similar gene expression patterns. These findings highlight multiple biological pathways whose expression patterns differ substantially between high and low IFN-α producers. These differences likely contribute to variations in anti-RV immunity and immunopathology between individuals.

2021-07-31 | GSE166292 | GEO

Transcriptomic analysis in human bronchial epithelial cells after rhinovirus (RV) and influenza (IAV) virus infection for 48 hours

Project description:RNA-seq analysis to study the antiviral effect induced by RV and influenza A virus

2022-03-01 | GSE193164 | GEO

Murine precision-cut liver slices (PCLS): a new tool for studying tumor microenvironments and cell signaling ex vivo.

Project description: Not available

| S-EPMC4226874 | biostudies-literature