Absolute quantification of cohesin, CTCF and their regulators in human cells

ABSTRACT: We have measured absolute copy numbers and dynamics of cohesin, CTCF and their regulators NIPBL, WAPL and sororin by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching in HeLa cells before and after DNA replication. Comparison of these numbers with chromatin immunoprecipitation-sequencing data implies that some genomic cohesin and CTCF enrichment sites are unoccupied in single cells at any one time.

OTHER RELATED OMICS DATASETS IN: PXD012712

ORGANISM(S): Homo sapiens

PROVIDER: GSE126990 | GEO | 2019/11/21

REPOSITORIES: GEO