Genomics

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Drosophila Trf4-1 involvement in mRNA and primary miRNA transcription


ABSTRACT: Drosophila Trf4-1 is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Overall design: Small RNA profile of RNAi based knock-down cells

INSTRUMENT(S): Illumina HiSeq 2000 (Drosophila melanogaster)

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: xi zhou  

PROVIDER: GSE127210 | GEO | 2019-05-01

REPOSITORIES: GEO

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