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Single cell RNA-seq identifies cell diversity in embryonic salivary glands [Bulk RNA-seq]

ABSTRACT: Our single-cell and bulk transcriptome analyses revealed striking molecular heterogeneity in the mesenchymal cells of embryonic submandibular and parotid salivary glands during bud initiation, including distinct mesodermal and neural crest-derived molecular signatures that foreshadow later functions.

ORGANISM(S): Mus musculus

PROVIDER: GSE127260 | GEO | 2019/10/25

REPOSITORIES: GEO

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Single cell RNA-seq identifies cell diversity in embryonic salivary glands [Single cell RNA-seq]

Project description:Our single-cell and bulk transcriptome analyses revealed striking molecular heterogeneity in the mesenchymal cells of embryonic submandibular and parotid salivary glands during bud initiation, including distinct mesodermal and neural crest-derived molecular signatures that foreshadow later functions.

2019-10-25 | GSE127469 | GEO

Three Major salivary glands

Project description:The aim of this study is characterize the gene expression of rat parotid, submandibular and sublingual glands, providing basic information for the salivary marker proteins.

2010-05-15 | GSE21843 | GEO

T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands

Project description:Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis. Toluidine blue-stained cryostat sections of embryonic day 12.5 submandibular salivary glands were used for laser microdissection using an LMD system (Leica) with a pulsed UV laser on an upright automated microscope. A total of approximately 500 cells from a region immediately adjacent to a forming cleft or from a peripheral end bud region were excised by the laser and transferred to caps of 0.5 ml PCR tubes. SAGE libraries were constructed from the microdissected cleft and bud epithelia cells. SAGE tags were identified using the National Center for Biotechnology Information (NCBI) tag map set for UniGene.

2010-06-16 | E-GEOD-22374 | biostudies-arrayexpress

Transcriptome Analysis of Rabies virus-infected Mice Salivary Glands

Project description:Rabies is a fatal zoonotic disease posing a threat to the public health globally. Rabies virus (RABV) is excreted in the saliva of infected animals, and is primarily transmitted through bite contact. Salivary glands play an important role for virus propagation. However, the significance of salivary glands is less studied in RABV pathogenic mechanisms. To identify functionally important genes in the salivary glands, we employed RNA sequencing (RNA-seq) to establish and analyze mRNA expression profiles in parotid tissue infected with two RABV strains, CVS-11 and PB4. We map the transcriptome changes in response to RABV infection in parotid tissue for the first time. This work provides new clues to the study of RABV-affected salivary gland function and RABV transmission mechanisms in parotid tissue. And the salivary gland-enriched transcripts could be potential targets of interest for rabies disease control.

2023-11-26 | GSE248365 | GEO

T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands

2010-06-17 | GSE22374 | GEO

Functional characterization of the Differences Between Parotid and Submandibular Salivary Glands

Project description:Functional characterization of the Differences Between Parotid and Submandibular Salivary Glands

| PRJNA543115 | ENA

Gene expression of murine 3 major salivary gland organoids

Project description:Salivary glands that produce and secret saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we established long-term murine salivary gland organoids from 3 major salivary glands, including parotid gland (PG), submandibular gland (SMG), and sublingual gland (SLG). Murine salivary gland organoids expressed gland-specific genes and proteins of acinar, myoepithelial, and duct cells. Organoids were maintained in growth media (named GEM) and further underwent differentiation in differentiation media (named DAM). Our study will provide an experimental platform for the exploration of mechanisms involvled in tissue regeneration, development, or several salivary gland diseases.

2022-05-05 | GSE184091 | GEO

Mapping differences in salivary gland secretions using dried saliva spots

Project description:Dried saliva spot (DSS) sampling is a minimally invasive extraction technique that allows for the spatial mapping of salivary protein distribution in the oral cavity. In conjunction with untargeted nanoLC-Orbitrap-MS/MS analysis, we used DSS to compare the unstimulated proteomes secreted by the parotid and submandibular/sublingual salivary glands

2019-09-04 | PXD012792 | Pride

Integrative analysis of transcriptome and proteome sheds light on functional differences between human salivary glands

Project description:Saliva aids in the predigestion of food and perception of taste. It helps to maintain the integrity of the mineralized tooth and epithelial surfaces in the mouth, and shields the oro-digestive tract from environmental hazards and invading pathogens. Although salivary glands and saliva fluid are biologically and functionally inseparable, they have thus far been investigated as separate entities. To bridge this gap, we performed an integrative analysis of the transcriptome of 27 samples collected from the major human adult and fetal major salivary glands - submandibular, sublingual, and parotid - along with mass-spectrometry-based saliva proteome data and immunohistochemical localization in glandular tissue. Our results suggest that functional maturation at the transcriptome level occurs late in gland development, and is driven mainly by the transcription of genes that code for secreted saliva proteins. We further provide evidence that protein dosage of the most abundant salivary proteins secreted by the salivary glands is predominantly regulated at the transcriptome level. Finally, we demonstrate distinct transcriptomic profiles of each major salivary gland type that reveal functional specialization and will aid in future clinical analyses. Our study provides the hitherto most comprehensive RNAseq dataset of healthy salivary glands in humans, thus establishing a robust framework for deeper studies of saliva and salivary gland biology, development, and evolution, ultimately paving the way for better understanding the importance of these craniofacial secretory organs in health and their malfunctions in disease.

2020-07-10 | GSE143702 | GEO

Gene expression analysis of human salivary gland and organoid at single cell resolution

Project description:Salivary glands that produce and secret saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. Maintenance of diverse salivary gland cells in organoids remains problematic. Here, we established human salivary gland organoids, which is composed of multiple cellular subsets, from 3 major salivary glands, including parotid gland (PG), submandibular gland (SMG), and sublingual gland (SLG). Human salivary gland organoids expressed gland-specific genes and proteins of acinar, myoepithelial, and duct cells. Organoids were maintained in growth media (named GEM) and further underwent differentiation in differentiation media (named DAM). Our study will provide an experimental platform for the exploration of mechanisms involvled in tissue regeneration, development, or several salivary gland diseases.

2022-05-05 | GSE184526 | GEO

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