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Dataset Information

Gene expression profiling of Arabidopsis floral homeotic mutants

ABSTRACT: The gene expression profiles of inflorescences of the floral homeotic mutants apetala1, apetala2, apetala3, pistillata, and agamous were compared to wild-type inflorescences using a flower-specific cDNA array (GSM21001-21015) and a "whole-genome" oligonucleotide array (Operon) (GSM21016-21035). All experiments were performed at least in triplicate. This SuperSeries is composed of the SubSeries listed below.

INSTRUMENT(S): Meyerowitz Lab Arabidopsis Flower-specific cDNA Array

SUBMITTER: Frank Wellmer

PROVIDER: GSE1275 | GEO | 2004-05-02

SECONDARY ACCESSION(S): GSE1271 GSE1267 GSE1268 GSE1272PRJNA87505 GSE1269 GSE1273 GSE1265 GSE1270S-EPMC2683881 GSE1274 GSE1266

REPOSITORIES: GEO

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Publications

Genome-wide analysis of spatial gene expression in Arabidopsis flowers.

Wellmer Frank F Riechmann José Luis JL Alves-Ferreira Márcio M Meyerowitz Elliot M EM

The Plant cell 20040420 5

We have compared the gene expression profiles of inflorescences of the floral homeotic mutants apetala1, apetala2, apetala3, pistillata, and agamous with that of wild-type plants using a flower-specific cDNA microarray and a whole genome oligonucleotide array. By combining the data sets from the individual mutant/wild type comparisons, we were able to identify a large number of genes that are, within flowers, predicted to be specifically or at least predominantly expressed in one type of floral ...[more]

PMID: 15100403

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Project description:We have further characterized floral organ-localized gene expression in the inflorescence of Arabidopsis thaliana by comparison of massively parallel signature sequencing (MPSS) data. Six libraries of RNA sequence tags from immature inflorescence tissues were constructed and matched to their respective loci in the annotated Arabidopsis genome. These signature libraries survey the floral transcriptome of wild-type tissue as well as the floral homeotic mutants, apetala1, apetala3, agamous, a superman/apetala1 double mutant, and differentiated ovules dissected from the gynoecia of wild-type inflorescences. Comparing and contrasting these MPSS floral expression libraries enabled demarcation of transcripts enriched in the petals, stamens, stigma-style, gynoecia, and those with predicted enrichment within the sepal/sepal-petals, petal-stamens, or gynoecia-stamens.By comparison of expression libraries, a total of 572 genes were found to have organ-enriched expression within the inflorescence. The bulk of characterized organ-enriched transcript diversity was noted in the gynoecia and stamens, whereas fewer genes demonstrated sepal or petal-localized expression. Validation of the computational analyses was performed by comparison with previously published expression data, in situ hybridizations, promoter-reporter fusions, and reverse transcription PCR. A number of well-characterized genes were accurately delineated within our system of transcript filtration. Moreover, empirical validations confirm MPSS predictions for several genes with previously uncharacterized expression patterns.This extensive MPSS analysis confirms and supplements prior microarray floral expression studies and illustrates the utility of sequence survey-based expression analysis in functional genomics. Spatial floral expression data accrued by MPSS and similar methods will be advantageous in the elucidation of more comprehensive genetic regulatory networks governing floral development.