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Characterization of Npas4 and heterodimer DNA binding in stimulated and silenced rat neurons

ABSTRACT: We evaluated genome-wide DNA binding of NPAS4, ARNT1, ARNT2, H3K27ac, and RNAPII by chromatin immunoprecipitation sequencing (ChIP-seq) in mature primary hippocampal neurons (rat, DIV 28) that were pharmacologically silenced (“–”; 24 hours in TTX, CPP, and NBQX) or silenced and then treated with PTX for two hours (“+”) to trigger the production of AP- and EPSP-induced NPAS4.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE127793 | GEO | 2019/10/02

REPOSITORIES: GEO

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Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons (array)

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