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Full-length transcriptome reconstruction reveals large diversity of RNA isoforms and open reading frames in rat hippocampus

ABSTRACT: We developed a hybrid-sequencing workflow, combining next-generation and third-generation sequencing, to reconstruct full-length transcriptomes. Integrating with polysome profiling and ribosome footprinting data, we predicted isoform–specific translational status and reconstructed ORFeome. Moreover, we identified isoforms with specific subcellular localization pattern in neurons.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE128136 | GEO | 2019/10/14

REPOSITORIES: GEO

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