Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Calibrated Scc1, Sgo1 and Brn1 ChIP-seq in metaphase with and without tension, in strains where convergent genes at pericentromere borderes are rearranged into a tandem orientation

ABSTRACT: Genome binding/occupancy profiling of Sgo1 and the condensin subunit Brn1 by high throughput sequencing. S. pombe is included as the calibration genome.

ORGANISM(S): Schizosaccharomyces pombe Saccharomyces cerevisiae

PROVIDER: GSE128227 | GEO | 2020/01/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Calibrated Sgo1 and Brn1 ChIP-seq for wild type strains arrested in metaphase without tension

Project description:Genome binding/occupancy profiling of Sgo1 and the condensin subunit Brn1 by high throughput sequencing. S. pombe is included as the calibration genome.

2020-01-13 | GSE128236 | GEO

Calibrated Scc1 ChIP-seq for wild type and chl4Δ strains arrested in metaphase with and without tension

Project description:Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing. S. pombe is included as the calibration genome.

2020-01-13 | GSE128238 | GEO

Calibrated Scc1 ChIP-seq for wild type and rad61Δ strains arrested in metaphase with and without tension

Project description:Genome binding/occupancy profiling of the cohesin subunit Scc1 by high throughput sequencing. S. pombe is included as the calibration genome.

2020-01-13 | GSE128225 | GEO

Sgo1 ChIP-seq for wild type cells arrested in metaphase with and without tension

Project description:Genome binding/occupancy profiling of Sgo1 by high throughput sequencing

2020-01-13 | GSE104009 | GEO

Calibrated Sgo1 and Brn1 ChIP-seq for wild type strains arrested in metaphase without tension

Project description:Calibrated Sgo1 and Brn1 ChIP-seq for wild type strains arrested in metaphase without tension

| PRJNA526856 | ENA

Calibrated Scc1, Sgo1 and Brn1 ChIP-seq in metaphase with and without tension, in strains where convergent genes at pericentromere borderes are rearranged into a tandem orientation

Project description:Calibrated Scc1, Sgo1 and Brn1 ChIP-seq in metaphase with and without tension, in strains where convergent genes at pericentromere borderes are rearranged into a tandem orientation

| PRJNA526837 | ENA

Molecular Basis for CPC-Sgo1 Interaction: Implications for Centromere Localisation and Function of the CPCMolecular Basis for CPC-Sgo1 Interaction: Implications for Centromere Localisation and Function of the CPC

Project description:Sgo1 makes multipartite interactions with CPC subunits Previous studies have suggested that the Sgo1-CPC (Chromosomal Passenger Complex) interaction is mediated via the N-terminal coiled-coil of Sgo1 and Borealin. However, our structural data revealed that the very N-terminus of Sgo1 can interact with the BIR domain of Survivin. Together, these studies suggest that multi-partite interactions between Sgo1 and different CPC subunits could facilitate CPC-Sgo1 complex formation. To gain further structural insights, we performed chemical cross-linking of the CPCISB10-280-Sgo11-415 complex using a zero-length cross-linker, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), followed by mass spectrometry analysis. Cross-linking-mass spectrometry (CLMS) data showed that: (1) consistent with our previous observations, the N-terminal region of Sgo1 (amino acids 1-34) makes extensive contacts with Survivin BIR domain (amino acids 18-89); (2) the N-terminal coiled-coil of Sgo1 (amino acids 10-120) interacts with the CPC triple helical bundle; (3) consistent with previous findings, the N-terminal coiled-coil also contacts the Borealin dimerisation domain; and (4) the Sgo1 region beyond the N-terminal coil-coil region, which is predicted to be unstructured, contacts both Survivin and Borealin with most contacts confined to the Sgo1 central region spanning amino acids (aa) 180-300. Thus, our cross-linking results suggests that Sgo1 interacts with CPC, mainly via two regions, the N-terminal coiled-coil domain and the unstructured central region.

2022-06-29 | PXD028433 | Pride

Sgo1 ChIP-seq for wild type cells arrested in metaphase with and without tension

Project description:Sgo1 ChIP-seq for wild type cells arrested in metaphase with and without tension

| PRJNA407933 | ENA

NGS-mRNA in lung tissues in RAG1-/- and in RAG1-/- Sgo1-/+ mice

Project description:We compared lung mRNA expression profiles between RAG1-/- control and RAG1-/- Sgo1-/+ chromosome instability model mice. RAG1-/-Sgo1-/+ mice developed significantly more spontaneous lung tumors.

2017-02-09 | GSE84383 | GEO

Brain RNAseq, comparing wild type and Sgo1 mouse

Project description:Brain RNAseq, comparing wild type and Sgo1 mouse (all 24 months of age)

2018-07-31 | GSE115185 | GEO