Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Histone crotonylation and its readers control virulence-related biology of a major human commensal and pathogen.

ABSTRACT: PAT-seq approach was utilised to determine the gene expression of C. albicans. Biological duplicates of wild type and taf14∆∆ mutant samples were grown to log phase, with or without calcofluor white treatment (20 µg/ml, 2 h).

ORGANISM(S): Candida albicans

PROVIDER: GSE128279 | GEO | 2020/04/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Histone crotonylation and its readers control virulence-related biology of a major human commensal and pathogen.

Project description:PAT-seq approach was utilised to determine the gene expression of C. albicans. Biological duplicates of wild type and taf14∆∆ mutant samples were grown to log phase, with or without crotonate treatment (50 mM, 2 h).

2020-04-20 | GSE141229 | GEO

Histone crotonylation and its readers control virulence-related biology of a major human commensal and pathogen.

Project description:Histone crotonylation and its readers control virulence-related biology of a major human commensal and pathogen.

| PRJNA592889 | ENA

Histone crotonylation and its readers control virulence-related biology of a major human commensal and pathogen.

Project description:Histone crotonylation and its readers control virulence-related biology of a major human commensal and pathogen.

| PRJNA526940 | ENA

Histone crotonylation links metabolism with gene expression

Project description:We performed RNA-seq across the yeast metabolic cycle (YMC) in wild-type and a strain containing a point mutant in TAF14 (W81A) that abolishes binding to acylated histones. We also performed ChIPseq using antibodies directed against histone H3K9acetyl, histone H3K9crotonyl, histone H3 across the wild-type YMC.

2019-08-28 | GSE120019 | GEO

Benomyl toxicity links histone H3 lysine 4 methylation to cell cycle control

Project description:The PAT-seq approach was utilised to determine the gene expression changes over the cell-cycle of wildtype and delta-set1 yeast strains. The cell were synchronised by alpha-factor arrest and cell-cycle release

2017-03-01 | GSE83162 | GEO

Differential gene expression in C. auris in presence of pyrvinium pamoate

Project description:PAT-seq approach was utilised to determine the gene expression in the the transcriptome of C. auris treated with the vehicle DMSO or drug pyrvvinium pamoate (PP). Biological triplicates of C. auris cultures grown in RPMI medium with either DMSO or 1uM PP for 30 minutes at 37 Celsius were havested and total RNA was isolated using standard procedures (hot phenol method).

2022-04-04 | GSE176354 | GEO

Transcriptomic landscapes provide a framework of native gene expression from which to imagine new connections in cell biology

Project description:The Poly(A)-Test RNA-seq, ( PAT-seq ) approach was utilized to determine gene expression and poly(A)-site change in S. cerevisiae during colony maturation.

2019-06-24 | GSE125804 | GEO

Association of Taf14 with acetylated histone H3 directs the DNA damage response and gene transcription

Project description:We sequenced mRNA from triplicate log-phase cultures of BY4741 (WT) transformed with pRS313-HA3-SSN6 and taf14D transformed with pRS313-HA3-SSN6 (empty vector), full-length pRS313-TAF14-HA3-SSN6, or pRS313-taf14W81A-HA3-SSN6 cultured in synthetic complete media lacking histidine. Examination of changes in gene expression when the YEATS domain of Taf14 is mutated so it cannot bind acetyl-H3.

2015-09-09 | E-GEOD-71768 | biostudies-arrayexpress

Taf2 mediates DNA binding of Taf14

Project description:The assembly and function of the yeast general transcription factor TFIID complex requires specific contacts between its Taf14 and Taf2 subunits, however, the mechanism underlying these contacts remains unclear. Here, we determined the molecular and structural basis by which the YEATS and ET domains of Taf14 bind to the C-terminal tail of Taf2 and identified a unique DNA-binding activity of the linker region connecting the two domains. We show that in the absence of ligands the linker region of Taf14 is occluded by the surrounding domains, and therefore the DNA binding function of Taf14 is autoinhibited. Binding of Taf2 promotes a conformational rearrangement in Taf14, resulting in a release of the linker for the engagement with DNA and the nucleosome. Genetic in vivo data indicate that the association of Taf14 with both Taf2 and DNA is essential for transcriptional regulation. Our findings provide a basis for deciphering the role of individual TFIID subunits in mediating gene transcription.

2022-08-12 | PXD033397 | Pride

In vivo commensal control of Clostridioides difficile virulence

Project description:We illustrate how metabolically distinct species of Clostridia can protect against or worsen Clostridioides difficile infection, modulating the pathogen's colonization, growth, and virulence to impact host survival. Gnotobiotic mice colonized with the amino acid fermenter Paraclostridium bifermentans survived infection while mice colonized with the butyrate-producer, Clostridium sardiniense, more rapidly succumbed. Systematic in vivo analyses revealed how each commensal altered the gut nutrient environment, modulating the pathogen's metabolism, regulatory networks, and toxin production. Oral administration of P. bifermentans rescued conventional mice from lethal C. difficile infection via mechanisms identified in specifically colonized mice. Our findings lay the foundation for mechanistically informed therapies to counter C. difficile disease using systems biologic approaches to define host-commensal-pathogen interactions in vivo.

2021-10-04 | GSE182613 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data