Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-sequencing of the female adult fly guts with enterocyte-specific overexpression and silencing of lark in control and Pseudomonas entomophila infected states.

ABSTRACT: The lark gene was either overexpressed or silenced in adult enterocytes. Flies were fed sucrose or Pseudomonas entomophila (Pe).

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE128488 | GEO | 2019/10/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-sequencing of the adult female fly guts (w1118) with and without oral infection with Pseudomonas entomophila (Pe) or Erwinia carotovora carotovora 15 (Ecc15)

Project description:Adult female Drosophila melanogaster flies from the w1118 background were fed sucrose, Pseudomonas entomophila (Pe), or Erwinia carotovora carotovora 15 (Ecc15).

2019-10-07 | GSE128489 | GEO

Pseudomonas virulence factor controls expression of virulence genes in Pseudomonas entomophila

Project description:Quorum sensing is a communication strategy that bacteria use to collectively alter gene expression in response to cell density. Pathogens use quorum sensing systems to control activities vital to infection, such as the production of virulence factors and biofilm formation. The Pseudomonas virulence factor (pvf) gene cluster encodes a quorum sensing system (Pvf) that is present in over 500 strains of proteobacteria, including strains that infect a variety of plant and human hosts. We have shown that the Pvf quorum sensing system regulates the production of secreted proteins and small molecules in the insect pathogen Pseudomonas entomophila L48. Here, we identified genes that are likely regulated by Pvf using the model strain P. entomophila L48 which does not contain other known quorum sensing systems. Pvf regulated genes were identified through comparing the transcriptomes of wildtype P. entomophila and a pvf deletion mutant (ΔpvfA-D). We found that deletion of pvfA-D affected the expression of approximately 300 genes involved in virulence, the type VI secretion system, siderophore transport, and branched chain amino acid biosynthesis. Additionally, we identified seven putative biosynthetic gene clusters whose expression are reduced in ΔpvfA-D. Our results indicate that Pvf controls multiple virulence mechanisms in P. entomophila L48. Characterizing genes regulated by Pvf will aid understanding of host-pathogen interactions and development of anti-virulence strategies against P. entomophila and other pvf-containing strains.

2023-03-19 | GSE216056 | GEO

Pseudomonas entomophila

Project description:Pseudomonas entomophila overview

| PRJNA35901 | ENA

Pseudomonas entomophila

Project description:Pseudomonas entomophila Genome sequencing

| PRJNA508466 | ENA

Pseudomonas entomophila strain:23S

Project description:Pseudomonas entomophila strain:23S Genome sequencing

| PRJNA669330 | ENA

Pseudomonas entomophila strain:1257

Project description:Pseudomonas entomophila strain:1257 Genome sequencing

| PRJNA508546 | ENA

RNA-sequencing of the female adult fly guts with enterocyte-specific overexpression and silencing of lark in control and Pseudomonas entomophila infected states.

Project description:RNA-sequencing of the female adult fly guts with enterocyte-specific overexpression and silencing of lark in control and Pseudomonas entomophila infected states.

| PRJNA527835 | ENA

Liposcelis entomophila

Project description:Liposcelis entomophila overview

| PRJNA267454 | ENA

Serratia entomophila

Project description:Serratia entomophila overview

| PRJNA188000 | ENA

RNA-sequencing of the female adult fly guts from 38 DGRP lines with and without oral infection with Pseudomonas entomophila

Project description:In order to gain insights into gut immunocompetence and its variation in a population, we performed RNA-sequencing of whole guts of 38 Drosophila Genetic Reference Panel strains.

2019-10-07 | GSE118142 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data