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Effect of cycloheximide in the co-translation mRNA degradation pattern in set2D and rrp6D strains

ABSTRACT: We investigated cryptic transcription start site usage, chromatin organization and post-transcriptional consequences in Saccharomyces cerevisiae. We used 5PSeq approach, which measures ribosome dynamics by sequencing the presence of co-translation mRNA degradation intermediates, to assess if cryptic transcripts are engaged in active translation. Here we assay the effect of Cycloheximide treatment (CHX) in the co-translational degradation profile of using strains where cryptic transcription is enhanced. We study the profiles in set2D and rrp6D, enhanced for the presence of chromatin-sensitive and RNA degradation-sensitive cryptic transcripts respectively.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE128599 | GEO | 2019/10/01

REPOSITORIES: GEO

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