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Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [GV]

ABSTRACT: We performed RNAseq analysis to determine the effect of MFN2 deletion on oocyte global gene expression profile. RNAseq revealed a total of 363 genes were significantly differentially expressed (p<0.05) in Mfn2-/- GV oocytes compared to WT (241 up-regulated and 122 down-regulated). Gene ontology (GO) analysis indicated significant over-representation of elements involved in regulation of embryonic development, cell death and survival, and cell morphology. Pathway analysis of top10 upregulated and downregulated annotated genes are listed. Our findings provide new insight into the role of MFN2 in the oocytes, and may help understand the potential mechanism of subfertility and reproductive aging associated with MFN2-deficiency.

ORGANISM(S): Mus musculus

PROVIDER: GSE128780 | GEO | 2019/09/30

REPOSITORIES: GEO

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Mitofusin 2 is essential for oocyte and follicle development, and to maintain ovarian follicular reserve during reproductive aging [SFO]

Project description:We performed RNAseq analysis to determine the effect of MFN2 deletion on oocyte global gene expression profile. RNAseq revealed a total of 1041 genes were significantly differentially expressed (p<0.05) in Mfn2-/- seceondary follicle enclosed oocytes (SFOs) compared to WT, with 510 up-regulated and 531 down-regulated genes. GO cluster analysis indicated significant over-representation of elements involved in regulation of cell death and survival, cellular development and cellular growth. Pathway analysis of top10 upregulated and downregulated annotated genes are listed. Our findings provide new insight into the role of MFN2 in the oocytes, and may help understand the potential mechanism of subfertility and reproductive aging associated with MFN2-deficiency.

2019-09-30 | GSE128808 | GEO

Quantitative proteomic analysis of both fresh and vitrified porcine GV oocytes during in vitro maturation

Project description:In this study, we used tandem mass tag (TMT)-based quantitative approach to acquire proteomic profiles of porcine GV and MII oocytes and MII oocytes vitrified at the GV stage.

2021-07-01 | PXD023107 | Pride

Transcriptome analysis of mouse Cnot6l-/- preovulatory oocytes (GV), ovulated eggs (MII), and 1-cell zygotes

Project description:We profiled transcriptomes from Cnot6l deadenylase knock-out mouse GV oocytes, MII eggs and 1-cell zygotes in order to analyse its function during the oocyte-to-embryo (OET) transition. Transcriptome of wild-type golden hamster GV oocytes was also profiled.

2018-07-10 | GSE116771 | GEO

Microarray profiling of UPF2-deficient mouse GV oocytes.

Project description:To study the role of UPF2 during oogenesis, Upf2 was conditionally deleted in growing oocytes. Total RNA samples from UPF2-deficient GV oocytes were subjected to array profiling. The control samples for this experiment can be found in E-MTAB-5056.

2019-01-14 | E-MTAB-7064 | biostudies-arrayexpress

TMT-based proteomics analysis of GV, GVBD and MII oocytes

Project description:Two replicates of GV, GVBD, and MII oocytes were subjected to the 6-plex TMT labeling, HP-RP fractionation, and LC-MS/MS analysis. Two labeling experiments were performed for the total four replicates of each stage of oocytes.

2022-02-15 | PXD018777 | Pride

Microarray data of prepubertal, peripubertal, and adult oocytes, and from GV and MII oocytes matured in vivo or in vitro

Project description:Expression data from prepubertal, peripubertal, and adult derived mouse oocytes, and from germinal vesicle (GV), in vivo matured, and in vitro matured mouse oocytes. Oocytes derived from prepubertal females, or oocytes matured in vitro, are less developmentally competent compared to adult derived, or in vivo matured, oocytes, indicated by decreased embryonic development. One potential mechanism for decreased developmental potetential in prepubertal or in vitro matured oocytes is inadequate or inappropriate RNA degradation during oocyte maturation (progression from GV to MII). To investigate mechanisms involved in establishing oocyte cytoplasmic maturation and developmental competence, Affymetrix GeneChip microarrays were used. Keywords: Oocyte developmental competence The study encompassed three experimental designs using female B6D2F1 mice: 1) In vitro matured oocytes were obtained from d20 (prepubertal), d26 (peripubertal), and 7-8 wk old (adult) mice; 2) in vivo and in vitro matured oocytes were obtained from d26 mice; and 3) GV, in vivo matured, and in vitro matured oocytes were obtained from 7-8 wk old mice. RNA was extracted from pools of 150 oocytes and hybridized onto the Affymetrix microarrays.

2010-07-12 | E-GEOD-12432 | biostudies-arrayexpress

Microarray data of prepubertal, peripubertal, and adult oocytes, and from GV and MII oocytes matured in vivo or in vitro

2010-07-12 | GSE12432 | GEO

Proteomic-based identification of oocyte maturation-related proteins in mouse GV oocytes

Project description:In this study, we applied 1D SDS-PAGE and RP-LC-MS/MS to investigate the proteins stored in GV mouse oocytes. This high-performance strategy allowed us to define a set of 1405 different mouse GV oocyte proteins. It is confirmed that this study will help us to understand the diverse biological processes occurring in mouse oocytes and during early embryo development. However, compared with proteomic analysis of other cells and tissues, such as embryonic stems from cells and liver, the proteins identified in mature mouse oocytes were limited. This was mainly due to the fact that oocytes obtained from each mouse were very limited. We believe that the catalog of maternal proteins presented in this article is a starting point and we anticipate that more researches on the oocyte proteome will deduce most of the maternal proteins.

2020-09-17 | PXD014314 | Pride

Contrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.

Project description:Poly-ribosomal mRNA from pools of 75 bovine oocytes at the germinal vesicle(GV) and metaphase II(MII) stages were isolated by sucrose fractionation then compared through microarray hybridization. 3 biological replicates from germinal vesicle(GV) and metaphase II(MII) bovine oocytes were hybridized against each other while alternating dyes. One additional technical replicate (dye-swap of one of the previous hybridizations) was added to balance dye usage. The normalized log2 ratio (GV/MII) data is provided in the 'normalized_data.txt' file. Please note that data were scanned/extracted using a different Agilent reference file, and thus the feature extraction number for a given probe (in both normalized and raw data files) is different from that in the official Agilent-041017 platform (GPL18537). The reference file used for this study is provided as a Series supplementary file (041017_D_DNAFront_BCBottom_20120517.txt).

2014-09-09 | E-GEOD-56603 | biostudies-arrayexpress

Contrast of poly-ribosomal mRNA in germina vesicle (GV) and metaphase II (MII) bovine oocytes.

2014-09-09 | GSE56603 | GEO

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