Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type (WT), AAG-/-, and ELP1-/- Transcriptomes

ABSTRACT: mRNA profiles of HEK293T wild type (WT), AAG-/-, and ELP1-/- cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.

OTHER RELATED OMICS DATASETS IN: PXD013508

ORGANISM(S): Homo sapiens

PROVIDER: GSE129009 | GEO | 2020/02/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type (WT), AAG-/-, and ELP1-/- Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type (WT), AAG-/-, and ELP1-/- Transcriptomes

| PRJNA529694 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and opaque2 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that opaque2 mutant influence the expression of storage proteins. Results provide important information of the gene expression level regulation of storage proteins and other biological processes. Endosperm mRNA profiles of 15DAP wild type (WT) and opaque2 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2014-10-15 | E-GEOD-61830 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the expression of translation and nucleosome assembly associated genes. Results provide important information of the gene expression level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes. Endosperm mRNA profiles of 15DAP wild type (WT) and rea1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2015-04-25 | E-GEOD-67080 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and dek35 mutant Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that dek35 mutant influence the expression of mitochondrial oxidative phosphorylation associated genes. Results provide important information of the gene expression level regulation of mitochondrial complexes assembly proteins, translational elongation factors and genes associated with other biological processes. Endosperm mRNA profiles of 18DAP wild type (WT) and dek35 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2016-03-25 | E-GEOD-79583 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant total and polysome-bound Transcriptomes

Project description:Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the translation level of translation and nucleosome assembly associated genes. Results provide important information of the gene translation level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes. Endosperm total and polysome-bound mRNA profiles of 15DAP wild type (WT) and rea1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

2015-04-25 | E-GEOD-67083 | biostudies-arrayexpress

ELP1 splicing correction reverses proprioceptive sensory loss in familial dysautonomia

Project description:Familial dysautonomia (FD) is a recessive neurodegenerative disease caused by a splice mutation in Elongator complex protein 1 (ELP1, also known as IKBKAP) which leads to variable skipping of exon 20 and to a drastic reduction of ELP1 levels in the nervous system. Clinically, many of the debilitating aspects of the disease are related to a progressive loss of proprioception, which leads to severe gait ataxia, spinal deformities and respiratory insufficiency due to neuromuscular incoordination. There is currently no effective treatment for FD and the disease is ultimately fatal. Development of a drug that targets the underlying molecular defect provides hope that the drastic peripheral neurodegeneration characteristic of FD can be halted. We demonstrate herein that the FD mouse, TgFD9; IkbkapΔ20/flox, recapitulates the proprioceptive impairment observed in individuals with FD, and we provide the in vivo evidence that postnatal correction of mutant ELP1 splicing promoted by the small molecule kinetin can rescue neurological phenotypes in FD. Daily administration of kinetin starting at birth improves sensory-motor coordination and prevents the onset of spinal abnormalities by stopping the loss of proprioceptive neurons. These phenotypic improvements correlate with increased levels of full length ELP1 mRNA and protein in multiple tissues including the peripheral nervous system (PNS). Our results show that postnatal correction of the underlying ELP1 splicing defect can rescue devastating disease phenotypes and is therefore a viable therapeutic approach for persons with FD.

2019-03-21 | GSE126155 | GEO

Expression data from 17-AAG resistance cell line

Project description:Develop the 17-AAG resistance human lung cancer cell line and used microarrays to observe the gene alternnation

2017-01-11 | GSE93384 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and snf[148] ovary Transcriptomes

Project description:Comparison of wild type and tumorous ovaries reveals that tumors inappropriately express a large number of testis genes mRNA profiles of ovaries from 0-24 hour virgin wild type (WT) and 3 day old snf[148] females were generated by deep sequencing, in duplicate, using Illumina HiSeq 2500.

2015-02-17 | E-GEOD-65932 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of healthy indiviual and HUWE1-promoted XLID Transcriptomes

Project description:mRNA profiles of healthy vs HUWE1 mutant cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 4000 (single read, 75bp).

2021-03-06 | GSE130551 | GEO

Aag glycosylase and impact on gene expression changes

Project description:We are investigating the transcriptional response of mice that are wt or null for Aag in response to alkylating agent MMS We used microarrays to detail the global programme of gene expression response due to MMS exposure in a DNA repair proficient or deficient mouse Keywords: timepoint

2009-02-04 | GSE11252 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data