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Transcriptomic analysis for response of EHEC to short chain fatty acids


ABSTRACT: Enterohaemorrhagic Escherichia coli (EHEC) is an emerging pathogen that causes diarrhea and heamolytic uremic syndrome. Expression of genes associated to pathogenicity is strictly regulated by environmental factors. Since short chian fatty acids (SCFAs) are present in intestinal tract which is a target of EHEC infection, we investigated the response of EHEC genes to SCFAs, such as acetate, propionate and butyrate. Keywords: Culture condition Overall design: 3 sets of comparison between transcription profiles in EHEC growing in the presence of acetate, propionate or butyrate against EHEC growing in the presence of NaCl. Labelling of cDNA and hybridization were performed twice with independently prepared RNAs.

INSTRUMENT(S): Osaka University E. coli O157 Sakai Oligo Array 5.5K ver2

ORGANISM(S): Escherichia Coli

SUBMITTER: Toru Tobe  

PROVIDER: GSE12903 | GEO | 2008-11-01

SECONDARY ACCESSION(S): PRJNA110903

REPOSITORIES: GEO

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