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RNA sequence for mammary gland of ICR mice at pregnant day 17 with supplementation of methionine (Met) alone or with methionyl-methionine (Met-Met) dipeptide.

ABSTRACT: Using RNA-seq to explore the mechanisms underlysing the effects of methionyl-methionine dipeptide (Met-Met) or free methionine (Met) supplementation in Met-restricted mice during pregnancy on mammogenesis and lactation.

ORGANISM(S): Mus musculus

PROVIDER: GSE129224 | GEO | 2022/04/02

REPOSITORIES: GEO

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			Action	DRS
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	GSE129224_Annotated_lncRNA_FPKM.xls.gz	Xls
	GSE129224_Novel_lncRNA_FPKM.xls.gz	Xls
	GSE129224_RAW.tar	Raw
	filelist.txt	Txt

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RNA sequence for mammary gland of ICR mice at pregnant day 17 with supplementation of methionine (Met) alone or with methionyl-methionine (Met-Met) dipeptide.

Project description:RNA sequence for mammary gland of ICR mice at pregnant day 17 with supplementation of methionine (Met) alone or with methionyl-methionine (Met-Met) dipeptide.

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Project description:In Staphylococcus aureus, de novo methionine biosynthesis is regulated by a unique hierarchical pathway involving stringent-response controlled CodY repression in combination with a T-box riboswitch and RNA decay. The T-box riboswitch residing in the 5' untranslated region (met leader RNA) of the S. aureus metICFE-mdh operon controls downstream gene transcription upon interaction with uncharged methionyl-tRNA. met leader and metICFE-mdh (m)RNAs undergo RNase-mediated degradation in a process whose molecular details are poorly understood. Here, we determined the secondary structure of the met leader RNA and found the element to harbor, beyond other conserved T-box riboswitch structural features, a terminator helix which is a target for RNase III endoribonucleolytic cleavage. As the terminator is a thermodynamically highly stable structure, it also forms posttranscriptionally in met leader/ metICFE-mdh read-through transcripts. Cleavage by RNase III releases the met leader from metICFE-mdh mRNA and initiates RNase J-mediated degradation of the mRNA from the 5'-end. Of note, metICFE-mdh mRNA stability varies over the length of the transcript with a longer lifespan towards the 3'-end. Corresponding variations in protein levels led us to hypothesize that coordinated RNA decay represents another level in the hierarchical methionine biosynthesis control network to adjust methionine biosynthesis enzyme amounts to current metabolic requirements.

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Another layer of complexity in Staphylococcus aureus methionine biosynthesis control: Unusual RNase III-driven T-box riboswitch cleavage determines met operon mRNA stability and decay

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