Genomics

Dataset Information

35

Dgpp-2 DGPP-regulated genes: Identification of diacylglycerol pyrophosphate-regulated genes in ABA signaling


ABSTRACT: rs05-06_dgpp - dgpp - 2. The specific plant phosphorylated form of phosphatidic acid (PA), diacyglycerol pyrophosphate (DGPP), was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA. Overall design: 4 dye-swap - CATMA arrays 5ml of 3 days-old suspension cells were incubated with ABA or DGPP 18:1 for 3hours under the conditions of culture. DGPP was emulsified by sonication for 1min 4 times at 4°C in 1ml of culture medium then added to 4ml of suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. DGPP is from Avanti Polar Lipids.

INSTRUMENT(S): URGV Arabidopsis thaliana 25K CATMA_v2.2

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Soubigou-Taconnat Ludivine  

PROVIDER: GSE12938 | GEO | 2008-09-27

SECONDARY ACCESSION(S): PRJNA110863

REPOSITORIES: GEO

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