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Analysis of gene expression in colonic epithelial cells following DSS treatment

ABSTRACT: Mice with and without IFNAR expression were treated with broad-spectrum antibiotics for more than 10 days, either infected with MNV or left uninfected and then treated with 3% DSS in drinking water for 6 days. Intestinal epithelial cells were isolated from the colon and sorted and RNA extracted for RNASeq analysis. We found that mice with IFNAR expression on IEC and infected with MNV had a gene expression signature of DNA repair similar that induced by IL-22 signaling.

ORGANISM(S): Mus musculus

PROVIDER: GSE129384 | GEO | 2019/04/06

REPOSITORIES: GEO

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RNA-seq analysis of colonic intestinal epithelial cells (IECs) isolated from naive wild-type C57BL/6 mice, DSS-treated wild-type C57BL/6 mice, Il10-/- C57BL/6 mice and Il10-/- C57BL/6 mice with IEC-specific Rabgef1 deletion

Project description:The aim of this study is to compare the transcriptomic profile of colonic intestinal epithelial cells (IECs) at steady-state, and in several models of colitis: in naive wild-type C57BL/6 mice, in wild-type C57BL/6 mice treated for 7 days with 3% dextran sodium sulfate (DSS) in drinking water, in Il10-/- C57BL/6 mice and Il10-/- C57BL/6 mice with IEC-specific Rabgef1 deletion. Colonic IECs were isolated and FACS-sorted, then subjected tu bulk RNA-seq.

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2009-05-08 | E-GEOD-15955 | biostudies-arrayexpress

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2015-02-08 | E-GEOD-35651 | biostudies-arrayexpress

Small RNA Sequencing facilitates quantitative analysis of miRNA expression in feces from mice during DSS-induced colitis and the recovery phase

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2021-01-28 | GSE165648 | GEO

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