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Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [iR_iHEC]

ABSTRACT: We inserted the inducible expression cassette of Runx1 into the Rosa26 locus of embryonic stem cells. Based on a modified protocol for HEC induction from PSC, we successfully generated iHEC Under the haematopoietic induction process (iR-HEC).

ORGANISM(S): Mus musculus

PROVIDER: GSE129444 | GEO | 2020/07/31

REPOSITORIES: GEO

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Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [single hematopoietic cells (iHEC)]

Project description:We identified that the coordinated expression of exogenous Runx1 and Hoxa9 during the haematopoietic induction process from mouse PSC resulted in a type of induced hemogenic endothelial cells (iHEC). The single iHEC can be robustly educated into induced haematopoietic progenitor cells (iHPC), which gave rise to induced T cells (iT cells) with abundant TCRαβ repertoire in vivo. We sorted the single iHEC and iHPC from day-14, day-17, day-21 cell products derived from 241 iR9-ES and performed single-cell RNA-Seq.

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Expression profiling of Notch constitutive activation in the liver of mice

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Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [iR_iHEC]

Project description:Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [iR_iHEC]

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Transcriptional profiling of early haematopoietic mesoderm and endoderm in differentiating human pluripotent stem cells.

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Expression profiling of Notch constitutive activation in the liver of mice

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