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Cross-species single-cell analysis of pancreatic ductal adenocarcinoma reveals cancer-associated fibroblasts expressing MHC class II

ABSTRACT: This study used 10X Genomics, single-cell RNA-sequencing to examine the cell types present in the KrasLSL-G12D; Trp53LSL-R172H; Pdx1-Cre (KPC) mouse model for pancreatic ductal adenocarcinoma. The study analyzed tumors from 4 different mice. For each tumor, we performed flow sorting to isolate all viable cells, and to isolate a fibroblast-enriched population of cells for single-cell RNA-seq to determine the transcriptomes of individual cells in KPC pancreatic ductal adenocarcinoma tumors.

ORGANISM(S): Mus musculus

PROVIDER: GSE129455 | GEO | 2019/06/19

REPOSITORIES: GEO

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