Similar Datasets
Project description:Purpose: Opium poppy is one of the most important medical plants and remains the only commercial resource of morphinan-based painkillers. However, little is known about its regulation mechanism in benzylisoquinoline alkaloids (BIAs) biosynthesis. Herein, the Transcriptome dataset of Opium poppy was constructed to identify the gene involved in its regulation mechanism in BIAs biosynthesis. Methods: Using Illumina HiSeq X Ten platform, 33 samples of Illumina transcriptome data from different tissues, growth phases and cultivars were constructed. Results: The high-quality transcripts were subsequent quantified with the short reads, and the expression of each unigenes among different samples was calculated by RPKM (the reads per kilobase per million mapped reads). Conclusions: These data provide a foundation of opium poppy transcriptome which may contribute to understand the regulation of BIAs biosynthesis.
2019-04-11 | GSE129567 | GEO
Project description:Purpose: Opium poppy is one of the most important medical plants and remains the only commercial resource of morphinan-based painkillers. However, little is known about its regulation mechanism in benzylisoquinoline alkaloids (BIAs) biosynthesis. Herein, the Transcriptome dataset of Opium poppy was constructed to identify the gene involved in its regulation mechanism in BIAs biosynthesis. Methods: Using Illumina HiSeq X Ten platform, 33 samples of Illumina transcriptome data from different tissues, growth phases and cultivars were constructed. Results: The high-quality transcripts were subsequent quantified with the short reads, and the expression of each unigenes among different samples was calculated by RPKM (the reads per kilobase per million mapped reads). Conclusions: These data provide a foundation of opium poppy transcriptome which may contribute to understand the regulation of BIAs biosynthesis.
2019-04-11 | GSE129566 | GEO
Project description:Purpose: Opium poppy is one of the most important medical plants and remains the only commercial resource of morphinan-based painkillers. However, little is known about its regulation mechanism in benzylisoquinoline alkaloids (BIAs) biosynthesis. Herein, the Transcriptome dataset of Opium poppy was constructed to identify the gene involved in its regulation mechanism in BIAs biosynthesis. Methods: Using Illumina HiSeq X Ten platform, 33 samples of Illumina transcriptome data from different tissues, growth phases and cultivars were constructed. Results: The high-quality transcripts were subsequent quantified with the short reads, and the expression of each unigenes among different samples was calculated by RPKM (the reads per kilobase per million mapped reads). Conclusions: These data provide a foundation of opium poppy transcriptome which may contribute to understand the regulation of BIAs biosynthesis.
2019-04-11 | GSE129565 | GEO
Project description:Transcriptome analysis of Opium poppy using Illumina HiSeq X Ten platform
| PRJNA531829 | ENA
Project description:BackgroundDastarcus helophoroides is known as the most valuable natural enemy insect against many large-body longhorned beetles. The molecular mechanism of its long lifespan and reproduction makes it a unique resource for genomic research. However, molecular biological studies on this parasitic beetle are scarce, and genomic information for D. helophoroides is not currently available. Thus, transcriptome information for this species is an important resource that is required for a better understanding of the molecular mechanisms of D. helophoroides. In this study, we obtained transcriptome information of D. helophoroides using high-throughput RNA sequencing.ResultsUsing Illumina HiSeq 2000 sequencing, 27,543,746 clean reads corresponding to a total of 2.48 Gb nucleotides were obtained from a single run. These reads were assembled into 42,810 unigenes with a mean length of 683 bp. Using a sequence similarity search against the five public databases (NR, Swiss-Prot, GO, COG, KEGG) with a cut-off E-value of 10(-5) using Blastx, a total of 31,293 unigenes were annotated with gene description, gene ontology terms, or metabolic pathways.ConclusionsTo the best of our knowledge, this is the first study on the transcriptome information of D. helophoroides. The transcriptome data presented in this study provide comprehensive information for future studies in D. helophoroides, particularly for functional genomic studies in this parasitic beetle.
| S-EPMC4076278 | biostudies-literature
Project description:BackgroundMethylation of cytosine in genomic DNA is a well-characterized epigenetic modification involved in many cellular processes and diseases. Whole-genome bisulfite sequencing (WGBS), such as MethylC-seq and post-bisulfite adaptor tagging sequencing (PBAT-seq), uses the power of high-throughput DNA sequencers and provides genome-wide DNA methylation profiles at single-base resolution. However, the accuracy and consistency of WGBS outputs in relation to the operating conditions of high-throughput sequencers have not been explored.ResultsWe have used the Illumina HiSeq platform for our PBAT-based WGBS, and found that different versions of HiSeq Control Software (HCS) and Real-Time Analysis (RTA) installed on the system provided different global CpG methylation levels (approximately 5% overall difference) for the same libraries. This problem was reproduced multiple times with different WGBS libraries and likely to be associated with the low sequence diversity of bisulfite-converted DNA. We found that HCS was the major determinant in the observed differences. To determine which version of HCS is most suitable for WGBS, we used substrates with predetermined CpG methylation levels, and found that HCS v2.0.5 is the best among the examined versions. HCS v2.0.12 showed the poorest performance and provided artificially lower CpG methylation levels when 5-methylcytosine is read as guanine (first read of PBAT-seq and second read of MethylC-seq). In addition, paired-end sequencing of low diversity libraries using HCS v2.2.38 or the latest HCS v2.2.58 was greatly affected by cluster densities.ConclusionsSoftware updates in the Illumina HiSeq platform can affect the outputs from low-diversity sequencing libraries such as WGBS libraries. More recent versions are not necessarily the better, and HCS v2.0.5 is currently the best for WGBS among the examined HCS versions. Thus, together with other experimental conditions, special care has to be taken on this point when CpG methylation levels are to be compared between different samples by WGBS.
| S-EPMC5217569 | biostudies-literature
Project description:To identify the molecular basis of genome-wide transcriptome analysis in induced opium poppy capsule tissues NimbleGen microarray (12X135 K element) analysis was performed. Fungal elicitor methyl jasmonate (MeJa) treatment was applied on capsules of opium poppy during 0, 3, and 12 h.
2014-02-21 | GSE55196 | GEO
Project description:To identify the molecular basis of genome-wide transcriptome analysis in induced opium poppy capsule tissues NimbleGen microarray (12X135 K element) analysis was performed. Fungal elicitor methyl jasmonate (MeJa) treatment was applied on capsules of opium poppy during 0, 3, and 12 h. Four sample sets were prepared for array analyses: i) control ii) 0 hour after treatment iii) 3 hour after treatment iv) 12 h after treatment
2014-02-21 | E-GEOD-55196 | biostudies-arrayexpress
Project description:The Illumina HiSeq platform has been a commonly used option for bacterial genome sequencing. Now the BGI DNA nanoball (DNB) nanoarrays platform may provide an alternative platform for sequencing of bacterial genomes. To explore the impact of sequencing platforms on bacterial genome assembly, quality assessment, sequence alignment, functional annotation, mutation detection, and metagenome mapping, we compared genome assemblies based on sequencing of cultured bacterial species using the HiSeq 2000 and BGISEQ-500 platforms. In addition, simulated reads were used to evaluate the impact of insert size on genome assembly. Genome assemblies based on BGISEQ-500 sequencing exhibited higher completeness and fewer N bases in high GC genomes, whereas HiSeq 2000 assemblies exhibited higher N50. The majority of assembly assessment parameters, sequences of 16S rRNA genes and genomes, numbers of single nucleotide variants (SNV), and mapping to metagenome data did not differ significantly between platforms. More insertions were detected in HiSeq 2000 genome assemblies, whereas more deletions were detected in BGISEQ-500 genome assemblies. Insert size had no significant impact on genome assembly. Taken together, our results suggest that DNBSEQ platforms would be a valid substitute for HiSeq 2000 for bacterial genome sequencing.
| S-EPMC10788345 | biostudies-literature
Project description:The Lycaenidae butterflies, Protantigius superans and Spindasis takanosis, are endangered insects in Korea known for their symbiotic association with ants. However, necessary genomic and transcriptomics data are lacking in these species, limiting conservation efforts. In this study, the P. superans and S. takanosis transcriptomes were deciphered using Illumina HiSeq 2500 sequencing. The P. superans and S. takanosis transcriptome data included a total of 254,340,693 and 245,110,582 clean reads assembled into 159,074 and 170,449 contigs and 107,950 and 121,140 unigenes, respectively. BLASTX hits (E-value of 1.0 × 10(-5)) against the known protein databases annotated a total of 46,754 and 51,908 transcripts for P. superans and S. takanosis. Approximately 41.25% and 38.68% of the unigenes for P. superans and S. takanosis found homologous sequences in Protostome DB (PANM-DB). BLAST2GO analysis confirmed 18,611 unigenes representing Gene Ontology (GO) terms and a total of 5259 unigenes assigned to 116 pathways for P. superans. For S. takanosis, a total of 6697 unigenes were assigned to 119 pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Additionally, 382,164 and 390,516 Simple Sequence Repeats (SSRs) were compiled from the unigenes of P. superans and S. takanosis, respectively. This is the first report to record new genes and their utilization for conservation of lycaenid species population and as a reference information for closely related species.
| S-EPMC4691156 | biostudies-literature