Project description:Analysis of serum starved prelamin A-accumulating hMSCs at gene expression level. The hypothesis tested in the present study was that prelamin A accumulation induces the dysregulation of genes that are essensial for cell survival under a stress condition such as serum starvation. The results provide important information about these genes and the functional categories that are dysregulated due to prelamin A accumulation in serum starved hMSCs.
Project description:Samples-WT Basal condition primary cortex cells; WT B27 Starved-Primary cortex cells starved overnight without B27 supplement media. WT AA Starved-Primary cortex cell starved without amino acid for 2 hours. WT AA Refed-Primary cortex cell refed for 1 hour after amino acid starvation. KO Basal-SLC38 Knockout Primary cortex cells starved overnight without B27 supplement media. KO B27 Starved-SLC38 Knockout Primary cortex cell starved without amino acid for 2 hours. KO AA starved-SLC38 Knockout Primary cortex cell refed for 1 hour after amino acid starvation. KO AA Refed-SLC38 Knockout Primary cortex cell refed for 1 hour after amino acid starvation.
Project description:HEK293T cells grown to confluence in media +10% fetal bovine serume. Media was removed and replaced with serum free media, and cultured for 3 days. RNA was harvested from day0 (serum supplemented), control, and day3 (serum starved) cultures, experiment.
Project description:RNA-binding proteins (RBPs) are an essential component of ribonucleoprotein complexes and are crucial for post-transcriptional regulation by regulatory sRNAs. Well-studied examples in different groups of bacteria include RBPs such as various ribosomal and RRM-domain containing proteins, or the RNA chaperones Hfq, ProQ and CsrA. However, the knowledge about RBPs and possible RNA chaperones in photosynthetic cyanobacteria is very limited, although there is extensive evidence for the presence of post-transcriptional regulation. Here, we analyzed cellular protein complexes by gradient profiling with or without RNase treatment (Grad-R) in Synechocystis sp. PCC 6803.
