Genomics

Dataset Information

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RNA sequencing of single LN cells sorted from virus-activated CD4 T clusters


ABSTRACT: PA-GFP mice were infected subcutaneously with either rVSV or rLCMV viruses. Two days upon infection, draining popliteal lymph nodes (LN) were photoactivated in areas containing Ag-specific CD4+ T cell clusters. Photoactivated cells were single-cell sorted and RNA sequencing performed to determine cell composition of activated CD4+ T cell clusters. Overall design: CFP+ Ag-specific CD4 T cells were transfered into PA-GFP mice, which were then infected in the footpad with either rVSV or rLCMV viruses. Two days upon infection, draining popliteal lymph nodes (LN) were photoactivated in areas containing Ag-specific CD4+ T cell clusters. LN were processed and photoactivated GFP+ cells were single-cell sorted in 384-well capture plates containing 2 μL of lysis solution and 10 barcoded poly(T) reverse-transcription (RT) primers for single-cell RNA-seq

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Ido Amit  

PROVIDER: GSE130009 | GEO | 2020-01-01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE130009_GSM4221946-GSM4221962_metadatatxt.txt.gz Txt
GSE130009_metadata.txt.gz Txt
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