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M6A modification of a 3′ UTR site reduces RME1 mRNA levels to promote meiosis

ABSTRACT: We performed m6A-seq on 5h meiotic yeast from WT and ime4-cat background (IP and input), and RNA-seq on total and polysome RNA of 5h meiotic yeast from WT, ime4-cat, and rme1-10 backgrounds

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE130104 | GEO | 2019/06/07

REPOSITORIES: GEO

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Gene expression profiles of WT and ime4-/- mutant yeast cells, under vegetative and meiosis-inducing conditions

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Project description:We studied the meiotic regulation of transcription, by comparing the mRNA level at time t=1h to t=8h after meiosis induction, to the mRNA level at time t=0h. Keywords: yeast meiotic time course, transcriptome For each time point, we measured the level of mRNA extracted at the time point to a mRNA meiotic mix composed of mRNA extracted at each time point. We normalize this ratio to the ratio measured at time t=0h and transform into log2ratios. The experiment is carried out in triplicates, from three independant cultures.

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