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Dgpp-DGPP regulated genes. Identification of diacylglycerol pyrophosphate regulated genes in ABA signaling.

ABSTRACT: rs05-06_dgpp - dgpp - The specific plant phosphorylated form of phosphatidic acid (PA), diacyglycerol pyrophosphate (DGPP) was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA. - Five ml of 3 days-old suspension cells was incubated with ABA or lipids for 3 h under the conditions of culture. Lipids were emulsified by sonication for 1 mi, four times, at 4°C, in one ml of culture medium then added to 4 ml suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. Dioleoyl PA and dioctanoyl PA are from Sigma, dioleoyl DGPP and dioctanoyl DGPP are from Avanti Polar Lipids. Keywords: treated vs untreated comparison

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE13019 | GEO | 2008/10/08

SECONDARY ACCESSION(S): PRJNA112559

REPOSITORIES: GEO

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dgpp-DGPP regulated genes. Identification of diacylglycerol pyrophosphate regulated genes in ABA signaling.

Project description:rs05-06_dgpp - dgpp - The specific plant phosphorylated form of phosphatidic acid (PA), diacyglycerol pyrophosphate (DGPP) was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA. - Five ml of 3 days-old suspension cells was incubated with ABA or lipids for 3 h under the conditions of culture. Lipids were emulsified by sonication for 1 mi, four times, at 4Â°C, in one ml of culture medium then added to 4 ml suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. Dioleoyl PA and dioctanoyl PA are from Sigma, dioleoyl DGPP and dioctanoyl DGPP are from Avanti Polar Lipids. Keywords: treated vs untreated comparison 5 dye-swap - CATMA arrays

2010-05-19 | E-GEOD-13019 | biostudies-arrayexpress

dgpp-2 DGPP-regulated genes: Identification of diacylglycerol pyrophosphate-regulated genes in ABA signaling

Project description:rs05-06_dgpp - dgpp - 2. The specific plant phosphorylated form of phosphatidic acid (PA), diacyglycerol pyrophosphate (DGPP), was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA. 4 dye-swap - CATMA arrays 5ml of 3 days-old suspension cells were incubated with ABA or DGPP 18:1 for 3hours under the conditions of culture. DGPP was emulsified by sonication for 1min 4 times at 4Â°C in 1ml of culture medium then added to 4ml of suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. DGPP is from Avanti Polar Lipids.

2010-05-19 | E-GEOD-12938 | biostudies-arrayexpress

Transcription profiling of Arabidopsis cell cultures treated with ABA or lipids to identify diacylglycerol pyrophosphate regulated genes in ABA signaling

Project description:Identification of diacylglycerol pyrophosphate regulated genes in ABA signaling.<br><br> The specific plant phosphorylated form of phosphatidic acid (PA), diacylglycerol pyrophosphate (DGPP) was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA.<br> Five ml of 3 days-old suspension cells was incubated with ABA or lipids for 3 h under the conditions of culture. Lipids were emulsified by sonication for 1 mi, four times, at 4°C, in one ml of culture medium then added to 4 ml suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. Dioleoyl PA and dioctanoyl PA are from Sigma, dioleoyl DGPP and dioctanoyl DGPP are from Avanti Polar Lipids.

2006-11-22 | E-MEXP-473 | biostudies-arrayexpress

dgpp-2 DGPP-regulated genes: Identification of diacylglycerol pyrophosphate-regulated genes in ABA signaling

2008-09-27 | GSE12938 | GEO

The Electrostatic Basis of Diacylglycerol Pyrophosphate-Protein Interaction.

Project description: Not available

| S-EPMC8774204 | biostudies-literature

Coevolution of hormone metabolism and signaling networks expands plant adaptive plasticity

Project description:Differential transcriptomic analysis of 10-day-old Arabidopsis seedlings treated with 50 Î¼M ABA, PA, or DPA. 10-day-old Arabidopsis seedlings grown on MS medium was treated with 50 Î¼M ABA, PA, or DPA. Total RNA was extracted from biological duplicates, Illumina RNAseq was performed on each sample.

2016-08-11 | E-GEOD-65739 | biostudies-arrayexpress

Coevolution of hormone metabolism and signaling networks expands plant adaptive plasticity

Project description:Differential transcriptomic analysis of 10-day-old Arabidopsis seedlings treated with 50 μM ABA, PA, or DPA.

2016-08-11 | GSE65739 | GEO

Next-generation sequencing facilitates quantitative analysis of miRNAs of cetuximab-resistant colorectal cancer (HCT116-R) cells

Project description:Total RNAs were isolated from three groups, including A group (0×10^9/mL PA-MSHA-treated HCT116 cells), B group (0.3×10^9/mL PA-MSHA-treated HCT116 cells) and C group (0.4×10^9/mL PA-MSHA-treated HCT116 cells). MiRNA sequencing was performed to assess differential expression using next-generation sequencing. The data showed that most miRNAs levels were different in A, B and C groups. Importantly, miR-7-5p was greatly increased in a dose-dependent manner.

2021-08-13 | GSE181843 | GEO

The characteristic of cholesterol metabolism-related gene expression in ACP, PCP and PA

Project description:To test whether high content of lipids was originated from ACP cells, we used an unbiased RNA sequencing (RNA-seq) transcriptome profiling approach to compare ACP, PCP and PA

2023-04-12 | GSE217717 | GEO

Quantitative transcriptomic analysis of abscisic acid-induced and reactive oxygen species-dependent expression changes and proteomic profiling in Arabidopsis suspension cells

Project description:Early rapid changes in response to the phytohormone abscisic acid (ABA) have been observed at the transcript level, but little is known how these transcript changes translate to changes in protein abundance under the same conditions. Here we have performed a global quantitative analysis of transcript and protein changes in Arabidopsis suspension cells in response to ABA using microarrays and quantitative proteomics. In summary, 3494 transcripts and 50 proteins were significantly regulated by ABA over a treatment period of 20–24 h. Abscisic acid also caused a rapid and strong increase in production of extracellular reactive oxygen species (ROS) with an average half-rise time of 33 sec. A subset of ABA-regulated transcripts were differentially regulated in the presence of the ROS scavenger dimethylthiourea (DMTU) as compared with ABA alone, suggesting a role for ROS in the regulation of these ABA-induced genes. Transcript changes showed an overall poor correlation to protein changes (r = 0.66). Only a subset of genes was regulated at the transcript and protein level, including known ABA marker genes. We furthermore identified ABA regulation of proteins that function in a branch of glucosinolate catabolism previously not associated with ABA signaling. The discovery of genes that were differentially regulated at the transcript and at the protein level emphasizes the strength of our combined approach. In summary, our dataset not only expands previous studies on gene and protein regulation in response to ABA, but rather uncovers unique aspects of the ABA regulon and gives rise to additional mechanisms regulated by ABA. Experimental Goals: 1. Identification of the set of genes that is regulated by Abscisic acid (ABA) in T87 suspension cells. 2. Test the effect of the H2O2 scavenger Dimethylthiourea (DMTU) on gene activation by ABA 3. Comaprison of changes in transcript and protein abundance in response to ABA

2011-04-07 | E-GEOD-23301 | biostudies-arrayexpress

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