Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (ChIP-Seq)

ABSTRACT: We identified a novel cofactor of PRC2, expresesd predominantly in the gonad, that lmits PRC2 enzymatic activity by impeding the interaction between the core complex and its accessory subunits.

ORGANISM(S): Homo sapiens

PROVIDER: GSE130230 | GEO | 2019/07/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (RNA-Seq)

Project description:We identified a novel cofactor of PRC2, expressed predominantly in the gonad, that limits PRC2 enzymatic activity by impeding the interaction between the core complex and the accessory subunits.

2019-07-15 | GSE130229 | GEO

GPIF constrains Polycomb Repressive Complex 2 activity in germ cells

Project description:The project aimed at identifying the cofactors regulating Polycomb complex PRC2 enzymatic activity in gonads. We used a knock-in mouse model where the enzymatic subunit of PRC2 (either EZH2 or EZH1) is tagged (Flag-tag) to purify PRC2 from mouse adult testis. This experiment revealed the existence of a new cofactor for PRC2 that we called GPIF (AU022751).

2019-07-22 | PXD012354 | Pride

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells.

Project description: Not available

| S-EPMC6710278 | biostudies-literature

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-07-15 | GSE130231 | GEO

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

Project description:EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells

| PRJNA534322 | ENA

Jarid2 regulates hematopoietic stem cell function by acting with Polycomb Repressive Complex 2

Project description:Polycomb Repressive Complex 2 (PRC2) has been shown to play a key role in hematopoietic stem and progenitor cell (HSPC) function. Analyses of mouse mutants harboring deletions of core components have implicated PRC2 in fine-tuning multiple pathways that instruct HSPC behavior, yet how PRC2 is targeted to specific genomic loci within HSPCs remains unknown. Here we use shRNA-mediated knockdown to survey the function of known PRC2 accessory factors in HSPCs by testing the competitive reconstitution capacity of transduced murine fetal liver cells. We find that similar to the phenotype observed upon depletion of core subunit Suz12, depleting Jarid2 enhances the competitive transplantation capacity of both fetal and adult, mouse and human HSPCs. Gene expression profiling revealed common Suz12 and Jarid2 target genes that are enriched for the H3K27me3 mark established by PRC2. These data implicate Jarid2 as an important component of PRC2 that has a central role in coordinating HSPC function. RNA-seq of jarid knockdown, suz knockdown and control from HSPC in 16 week old mice.

2015-04-02 | E-GEOD-60808 | biostudies-arrayexpress

Dynamic protein interactions of the Polycomb Repressive Complex 2

Project description:Polycomb proteins assemble to form complexes with important roles in epigenetic regulation. The Polycomb Repressive Complex 2 (PRC2) modulates the di- and tri-methylation of lysine 27 on histone H3, each of which are associated with gene repression. Although three subunits, EZH1/2, SUZ12 and EED, form the catalytic core of PRC2, a wider group of proteins associate with low stoichiometry. This raises the question of whether dynamic variation of the PRC2 interactome results in alternative forms of the complex during differentiation. Here we compared the physical interactions of PRC2 in undifferentiated and differentiated states of NTERA2 pluripotent embryonic carcinoma cells. Label-free quantitative proteomics was used to assess endogenous immunoprecipitation of the EZH2 and SUZ12 subunits of PRC2. A high stringency dataset reflecting the endogenous state of PRC2 was produced that included all previously reported ‘core’ and ‘associated’ PRC2 components, and several novel interacting proteins. Comparison of the interactomes obtained in undifferentiated and differentiated cells revealed candidate proteins that favoured either one or other state. For example, SALL4 and ZNF281 associate with PRC2 in pluripotent cells, while PCL1 and SMAD3 more associate with PRC2 in differentiating cells. Analysis of the mRNA and protein levels of these factors found that their association with PRC2 correlated with their cell state-specific expression. Taken together, we propose that dynamic changes to the PRC2 interactome during differentiation may contribute to directing its activity during cell fate transitions.

2016-09-27 | PXD004462 | Pride

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (RNA-Seq)

Project description:EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (RNA-Seq)

| PRJNA534325 | ENA

EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (ChIP-Seq)

Project description:EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells (ChIP-Seq)

| PRJNA534326 | ENA

Dynamic protein interactions of the Polycomb Repressive Complex 2

2017-03-29 | MSV000080739 | MassIVE