Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Total RNA sequencing of prospective axial stem cell cultures derived from human pluripotant stem cells

ABSTRACT: Here we describe a protocol for converting pluripotent stem cells to stable bi/multipotent stem cell populations with axial progenitor characteristics. To analyse the global transcriptome of the derived lines, we performed RNA-sequencing of 3 different axial stem cell lines

ORGANISM(S): Homo sapiens

PROVIDER: GSE130281 | GEO | 2019/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Total RNA sequencing of prospective axial stem cell cultures derived from human pluripotant stem cells

Project description:Total RNA sequencing of prospective axial stem cell cultures derived from human pluripotant stem cells

| PRJNA534521 | ENA

RNA sequencing analysis of human embryonic stem cells and axial progenitors

Project description:We analysed the transcriptomes of human embryonic stem cells (hESCs) and in vitro derived axial progenitors.

2018-08-13 | GSE110608 | GEO

Greb1 is required for axial elongation and segmentation in vertebrate embryos

Project description:This study presents transcription profiles for mouse axial progenitors, presomitic mesoderm and tailbud mesoderm. During vertebrate embryonic development, the formation of axial structures is driven by a population of stem-like cells (axial progenitors) that reside in a region of the tailbud called the chordoneural hinge (CNH) where. We have compared the CNH transcriptome with those of surrounding tissues and shown that the CNH and tailbud mesoderm are transcriptionally similar, and distinct from the presomitic mesoderm. Amongst CNH-enriched genes are several that are required for axial elongation, including Wnt3a, Cdx2, Brachyury/T and Fgf8, and androgen/estrogen receptor nuclear signalling components such as Greb1.

2020-01-01 | GSE141519 | GEO

RNA sequencing analysis of human embryonic stem cells and axial progenitors

Project description:RNA sequencing analysis of human embryonic stem cells and axial progenitors

| PRJNA434099 | ENA

Axial Elongation of Caudalized Human Pluripotent Stem Cell Organoids Mimics Neural Tube Development [scRNA-Seq]

Project description:During mammalian embryogenesis, axial elongation of the neural tube is critical for establishing the anterior-posterior body axis, but is difficult to interrogate directly because it occurs post-implantation. Here we report an organoid model of neural tube extension using human pluripotent stem cell (hPSC) aggregates that recapitulates the morphologic and temporal gene expression patterns of neural tube development. Axially extending organoids consisted of longitudinally elongated epithelial compartments and contained TBXT(+)SOX2(+) neuromesodermal progenitors, PAX6(+) Nestin(+) neural progenitor populations, and MEOX1(+) paraxial mesoderm populations. Wnt agonism stimulated axial extensions in a dose-dependent manner and elongated organoids displayed regionalized rostral-caudal HOX gene expression, with hindbrain (HOXB1) expression distinct from brachial (HOXC6) and thoracic (HOXB9) expression. CRISPR interference-mediated silencing of BMP inhibitors induced elongation phenotypes that mimicked murine knockout models, and knock-down of the downstream Wnt target, TBXT, increased neuroepithelial compartmentalization and resulted in multiple extensions. These results indicate the potent morphogenic capacity of hPSC organoids to undergo axial elongation in a manner that can be used to dissect the cellular organization and patterning decisions that dictate early human nervous system development.

2021-05-28 | GSE155383 | GEO

Axial Elongation of Caudalized Human Pluripotent Stem Cell Organoids Mimics Neural Tube Development [RNA-Seq]

2021-05-28 | GSE155382 | GEO

Proteomics analysis of plasma from radiographic axial spondyloarthritis patients.

Project description:Tandem mass spectrometry analysis of proteins from depleted plasma from radiographic axial spondyloarthritis patients pre- and post-treated with adalimumab.

2024-05-02 | PXD026189 | Pride

Axial progenitors generate trunk neural crest cells in vitro

Project description:The in vitro generation of neural crest (NC) cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology and isolate NC derivatives for disease modelling/regenerative medicine applications. However, conventional differentiation protocols induce only a modest yield of NC cells corresponding to the trunk level. Here we show that trunk NC cells and, their downstream derivatives, sympathoadrenal progenitors, can be produced at a high efficiency from hPSC-derived axial progenitors, the in vitro counterparts of the posteriorly-located drivers of embryonic axis elongation. Moreover, using transcriptome analysis, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities. Collectively, our findings indicate that a post-cranial NC state is achieved through two different routes: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas a trunk fate relies on a posterior axial progenitor intermediate.

2018-08-10 | GSE109267 | GEO

RNA sequencing of control and TBXT-depleted human NMP-like axial progenitors

Project description:A Tetracycline (Tet)-inducible TBXT shRNA human embryonic stem cell (hESC) line was treated with WNT and FGF agonists in the presence and absence of Tet for three days to generate NMP-like axial progenitors

2021-09-23 | GSE184620 | GEO

Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids

Project description:Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids

| PRJNA415967 | ENA