Gene expression in human adipose tissue derived stromal cells (ADSC) after exposure to hypoxia
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ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE130393 | GEO | 2019/04/27
REPOSITORIES: GEO
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2010-06-18 | E-GEOD-22429 | biostudies-arrayexpress
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2013-06-11 | E-GEOD-47741 | biostudies-arrayexpress
Project description:Gene expression in human adipose tissue derived stromal cells (ADSC) after exposure to hypoxia
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Project description:Analysis of 20S proteasome in various tissue, adipose derived stem cells(ADSC) or HeLa cells treated with interferon-gamma. This results are compared with LC-SRM results obtained on the same samples. Global proteomics on ADSC amplified in 5% or 20% O2.
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Project description:Adipose-derived stromal cells (ADSCs) represent a promising stem cell source for tissue engineering and cell based therapy. However, long-term in vitro expansion of ADSCs impede stemness maintaining, partly attributed to deprivation of their original microenvironment. Incompetent cells largely limit the therapeutic effects in ADSCs based clinical use. Therefore, reconstructing a more physiologically and physically relevant niche is an ideal strategy to settle this issue and therefore facilitates their extensive applications in clinical. Here, we transplanted separated ADSCs to local subcutaneous adipose tissues of nude mice as an in vivo cell culture model. We found transplanted ADSCs maintained the primitive morphologies, showed improved proliferation and delayed senescence when compared to cells in conventional incubator. Significantly increased expression of stemness-related markers and multi-lineage differentiation abilities were further observed in in vivo cultured ADSCs. At last, sequencing results revealed different expression genes between ADSCs obtained from in vivo and in vitro conditions mainly located in extracellular matrix and extracellular space, which participated in regulating transcription and protein synthesis. Moreover, we found an Egr1 involved signaling pathway might exert a crucial impact on controlling stemness properties. Our findings might collectively pave the way between the bench and beside in regard of ADSCs based application.
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