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Genome wide CRISPR screen for Venetoclax resisatnce in MOLM13 cells using Brunello library

ABSTRACT: MOLM13 cells were infected with CRISPR/Cas9 library, selected for puromycn resistance for integration events and exposed to Venetoclax or vehicle, DMSO, at 1microMolar concentrations. Cells were collected at time 0 (post puromycin selection), day 7 and 14 , DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Hiseq using 6 samples per lane.

ORGANISM(S): Homo sapiens

PROVIDER: GSE130413 | GEO | 2019/04/30

REPOSITORIES: GEO

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