High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [GM12878]

ABSTRACT: While recent technical advancements have facilitated the mapping of epigenomes at the single-cell resolution, the throughput and quality of these methods have limited the widespread application of these technologies. Here, we describe a high-throughput platform for single-cell assay for transposase accessible chromatin (scATAC-seq) using droplet microfluidics that yields essential qualities for high-throughput profiling, including improvements in per-cell library complexity and throughput. This approach enables robust cell-typing of complex tissues and reveals a de novo atlas of cellular and epigenomic diversity across many cell types and cell stages.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE130537 | GEO | 2019/05/02

REPOSITORIES: GEO