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MSCs Exposed to Keratinocyte Condition Medium

ABSTRACT: In the present study, we demonstrate that hMSCs migrate toward human keratinocytes as well as toward conditioned medium from cultured human keratinocytes (KCM) indicating that the hMSCs can respond to signals from keratinocytes. Incubation of hMSCs with KCM induced dermal myofibroblast like differentiation characterized by expression of cytoskeletal markers vinculin and F-actin filaments with increased expression of alpha smooth muscle actin. We then examined the therapeutic efficacy of hMSCs in wound healing in two animal models representing normal and chronic wound healing. Accelerated wound healing, as determined by quantitative measurements of wound area, was observed when hMSCs and KCM exposed hMSCs (KCMSCs) were injected near the site of incisional/excisional wounds in nondiabetic athymic and NOD/SCID mice as compared with normal human fetal lung fibroblast WI38 cells or saline control induced wound healing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE13073 | GEO | 2009/06/30

SECONDARY ACCESSION(S): PRJNA109693

REPOSITORIES: GEO

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Transcription profiling of human mesechymal stem cells exposed to keratinocyte conditioned medium

Project description:In the present study, we demonstrate that hMSCs migrate toward human keratinocytes as well as toward conditioned medium from cultured human keratinocytes (KCM) indicating that the hMSCs can respond to signals from keratinocytes. Incubation of hMSCs with KCM induced dermal myofibroblast like differentiation characterized by expression of cytoskeletal markers vinculin and F-actin filaments with increased expression of alpha smooth muscle actin. We then examined the therapeutic efficacy of hMSCs in wound healing in two animal models representing normal and chronic wound healing. Accelerated wound healing, as determined by quantitative measurements of wound area, was observed when hMSCs and KCM exposed hMSCs (KCMSCs) were injected near the site of incisional/excisional wounds in nondiabetic athymic and NOD/SCID mice as compared with normal human fetal lung fibroblast WI38 cells or saline control induced wound healing. Experiment Overall Design: Keratinocyte conditioned media exposed MSCs(KCMSCs) were used in the experiment in triplicates as follows; Experiment Overall Design: KCM_1 Experiment Overall Design: KCM_2 Experiment Overall Design: KCM_3 Experiment Overall Design: Where as MSCs were exposed to Keratinocyte growth media for 30 days were used as a controls as follow; Experiment Overall Design: KGM_1 Experiment Overall Design: KGM_2 Experiment Overall Design: KGM_3 Experiment Overall Design: All the samples were analyzed.

2009-07-04 | E-GEOD-13073 | biostudies-arrayexpress

Analysis of the expression profiles of back skin and excisional wound-derived fibroblasts FACS-sorted from mice overexpressing activin A.

Project description:Overexpression of activin A by keratinocytes accelerates excisional wound healing in mice. Activin-promoted wound healing is mediated via the stroma, specifically by the wound immune cells and fibroblasts. To determine if activin A alters the gene expression profile of wound fibroblasts, we performed RNA-sequencing of fibroblasts FACS-sorted from excisional skin wounds of activin overexpressing mice. We found that activin induces a pro-fibrotic gene expression profile of these fibroblasts, leading to the upregulation of genes involved in collagen biosynthesis and remodeling.

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Transcription profiling by array of excisional biopsy wounds from young and old human subjects to measure the influence of age on cutaneous wound healing

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2008-04-10 | E-MEXP-1074 | biostudies-arrayexpress

microRNA expression in skin and wound edge tissue at different time points

Project description:Change in miRNA expression pattern during different phases of wound healing following full thickness excisional wounding was studied.

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Effects of mycophenolic acid on human fibroblast proliferation, migration and adhesion in vitro and in vivo

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microRNA expression in skin and wound edge tissue at different time points

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2014-03-06 | E-GEOD-55661 | biostudies-arrayexpress

Mus musculus

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2016-07-29 | E-GEOD-74558 | biostudies-arrayexpress

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Project description:We perfomed RNA-seq and wound healing analysis to identify the mechanism how SOX2 promote wound healing. We showed that induction of SOX2 in skin keratinocytes accelerates cutaneous wound healing by promoting keratinocyte migration and proliferation, and enhancement of angiogenesis via the upregulation of EGFR ligands.

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A novel epidermal-specific role for arginase1 during cutaneous wound repair

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2021-07-07 | E-MTAB-10213 | biostudies-arrayexpress

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