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Next Generation Sequencing Analysis of Beta-actin knockout (KO) and wildtype (WT) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Under Adipogenic Induced Conditions

ABSTRACT: mRNA profiles of beta-actin knockout (KO) and wild-type (WT) MEFs induced to adipocyte-like feature to study how global transcriptome changes during differentiation

ORGANISM(S): Mus musculus

PROVIDER: GSE130765 | GEO | 2019/05/07

REPOSITORIES: GEO

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Next Generation Sequencing Analysis of chemically reprogrammed osteoblasts from Beta-actin Wildtype (WT) Heterozygotes (HET) and knockout (KO) Mouse Embryonic Fibroblast (MEFs)

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Next Generation Sequencing Analysis of Beta-actin knockout (KO) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Stimulated by Viral Mimics

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Next Generation Sequencing Analysis of Beta-actin knockout (KO) and wildtype (WT) Mouse Embryonic Fibroblast (MEFs) Transcriptomes Under Adipogenic Induced Conditions

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RNAseq from Mfn1/2 knockout MEFs with adipogenic differentiation

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Gene expression profiles in mouse embryonic fibroblasts (MEFs) derived from BCL11B-KO mice

Project description:The differentiation of preadipocytes into adipocytes is controlled by several transcription factors, including peroxisome proliferator-activated receptor Î³ (PPARÎ³) and CCAAT/enhancer-binding protein Î± (C/EBPÎ±), which are known as master regulators of adipogenesis. BCL11B is a zinc finger-type transcription factor that regulates the development of the skin and central nervous and immune systems. Here, we found that BCL11B was expressed in the white adipose tissue (WAT), particularly the subcutaneous WAT and that BCL11Bâ/â mice had a reduced amount of subcutaneous WAT. During adipogenesis, BCL11B expression transiently increased in 3T3-L1 preadipocytes and mouse embryonic fibroblasts (MEFs). The ability for adipogenesis was reduced in BCL11B knockdown 3T3-L1 cells and BCL11Bâ/â MEFs, whereas the ability for osteoblastogenesis was unaffected in BCL11Bâ/â MEFs. Luciferase reporter gene assays revealed that BCL11B stimulated C/EBPÎ² activity. Furthermore, the expression of downstream genes of the Wnt/Î²-catenin signaling pathway was not suppressed in BCL11Bâ/â MEFs during adipogenesis. Thus, this study identifies BCL11B as a novel regulator of adipogenesis, which works, at least in part, by stimulating C/EBPÎ² activity and suppressing the Wnt/Î²-catenin signaling pathway. MEFs were derived from embryonic day 12.5 BCL11B+/+ and BCL11Bâ/â C57BL6 mice embryos. After 2 times passage, MEFs were differentiated using the adipocyte differentiation medium and 10 Î¼M pioglitazone. After 12 h of adipocyte differentiation, gene expression profiles were analyzed by DNA microarray.

2016-09-05 | E-GEOD-83980 | biostudies-arrayexpress

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