Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IGH VDJ-RNA-seq of circulating plasma cells at day zero and seven relative to experimental vaccination dosing

ABSTRACT: IGH VDJ-RNA-sequencing of CD19+IgD-CD27+CD71+CD38+CD20- circulating plasma cells prior to vaccination (day 0) and seven (day 63) days after the third vaccination.

ORGANISM(S): Homo sapiens

PROVIDER: GSE131090 | GEO | 2019/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

IGH VDJ-RNA-seq of circulating plasma cells at day zero and seven relative to experimental vaccination dosing

Project description:IGH VDJ-RNA-seq of circulating plasma cells at day zero and seven relative to experimental vaccination dosing

| PRJNA542644 | ENA

RNA-seq of circulating Tfh like cells at day zero and seven and 28 relative to experimental vaccination dosing

Project description:Total RNA-sequencing on 150-200 ICOS+CD38+ cTfh cells per person prior to vaccination (day 0), and seven (day 63) and 28 (day 84) days after the third vaccination.

2019-06-01 | GSE131088 | GEO

Igh repertoire analysis in primary pro-B cells from WT and NE1-/- mice (VDJ-seq)

Project description:Analysis of the antibody repertoire composition is now possible using VDJ-seq. We used this recently developed method for unbiased amplification from genomic DNA (gDNA) to directly compare the Igh repertoire of C57Bl/6 (WT) and NE1-/- pro-B cells. We find that a group of contiguous proximal and intermediate VH genes are under-utilized in V->DJ rearrangement in the absence of NE1 revealing a NE1 zone of influence. We report the VH gene usage profile from WT and NE1-/- primary pro-B cells from the VDJ-seq data.

2022-12-22 | GSE203484 | GEO

RNA-seq of circulating Tfh like cells at day zero and seven and 28 relative to experimental vaccination dosing

Project description:RNA-seq of circulating Tfh like cells at day zero and seven and 28 relative to experimental vaccination dosing

| PRJNA542640 | ENA

Unbiased quantification of immunoglobulin diversity at the DNA level with VDJ-seq

Project description:For high-throughput sequencing and quantification of immunoglobulin repertoires, most methodologies utilise RNA. However, output varies enormously between recombined genes due to different promoter strengths and differential activation of lymphocyte subsets, precluding quantitation of recombinants on a per cell basis. To date, DNA-based approaches have used V gene primer cocktails, with substantial inherent biases. Here we describe VDJ-seq, which accurately quantitates immunoglobulin diversity at the DNA level in an unbiased manner. This is accomplished with a single primer extension step using biotinylated J gene primers. By addition of unique molecular identifiers (UMI) before primer extension, we reliably remove duplicate sequences and correct for sequencing and PCR errors. Furthermore, VDJ-seq captures productive and non-productive VDJ and DJ recombination events on a per cell basis. Library preparation takes 3 days, with 2 days of sequencing, and 1 day of data processing and analysis.

2017-12-16 | GSE106458 | GEO

RNA-seq of circulating Tfh like cells at day zero and seven relative to seasonal influenza vaccination

Project description:RNA sequencing of 200 ICOS+CD38+CXCR5+PD-1+ cTfh cells immediately prior to, and seven days after influenza vaccination in four individuals

2019-06-01 | E-MTAB-7955 | biostudies-arrayexpress

Rhesus and cynomolgus IGH database of germline VDJ

Project description:Rhesus and cynomolgus IGH genotyping yields comprehensive databases of germline VDJ alleles for in-depth immunological studies

| PRJEB38839 | ENA

Transcription of productive and nonproductive VDJ-recombined alleles after IgH allelic exclusion.

Project description: Not available

| S-EPMC2230841 | biostudies-other

RNA-seq of circulating Tfh like cells at day zero and seven relative to seasonal influenza vaccination

Project description:RNA-seq of circulating Tfh like cells at day zero and seven relative to seasonal influenza vaccination

| PRJEB32530 | ENA

Single-cell RNAseq of phenotypically defined human B cell subsets

Project description:Separation of B cells has been historically important in discovering their functional relevance, particularly in relation to infection, immune disorders and vaccination. Traditional use of phenotypic markers often poses problems in distinguishing heterogeneous populations such as the Double Negative (DN, CD19+CD27-IgD-) cells. B cells represent a small subset of PBMCs; this represents challenges to use bottom-up approaches such as single-cell transcriptomics in defining B cell subpopulations. In this study we therefore used the 10X single-cell RNAseq platform on B cell populations already defined by FACS sorting (Transitional, CD19+CD27-IgD+CD10+; Naïve, CD19+CD27-IgD+CD10-; Classical Memory, CD19+CD27+IgD-; IgM Memory, CD19+CD27+IgD+; and DN). These data match known phenotypes to transcriptionally defined B cell subpopulations, and provide a reference atlas for researchers interested in better defining B cell subsets in their data.

2021-03-19 | E-MTAB-9544 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data