Transcriptomics,Genomics

Dataset Information

25

Zea mays Transcriptome or Gene expression


ABSTRACT: MuDR/Mu are a highly active transposon family moving by either cut only (or cut-and-paste) in strictly somatic tissues or net replicative transposition (absence of excision alleles) in reproductive tissues. Aside from the MuDR-encoded MURA and MURB proteins, other factors required for Mu transposition, particularly those contributing to the developmentally specific behavior, have yet to be identified. To address this question and assess the impact of a highly active transposon on the transcriptome, RNA was extracted from anthers at three developmental stages in Mu-active and -inactive stocks and compared on a 44,000 element oligonucleotide array. We found that approximately 30,000 unique genes are expressed at each stage. Of the ~10% (ca. 3000) differentially regulated transcripts per stage, there was approximately equal representation by the active or inactive individuals. Keywords: anther development, maize, Mu, transposon Overall design: Dye swap design with 4 replicates comparing Mu-active to -inactive lines at 3 anther stages. Spike-in controls were included.

INSTRUMENT(S): Agilent-016047 Walbot Zea mays 4x44K array

SUBMITTER: John F Fernandes  

PROVIDER: GSE13118 | GEO | 2009-05-01

SECONDARY ACCESSION(S): PRJNA109655

REPOSITORIES: GEO

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Publications

Transcriptomes and proteomes define gene expression progression in pre-meiotic maize anthers.

Zhang Han H   Egger Rachel L RL   Kelliher Timothy T   Morrow Darren D   Fernandes John J   Nan Guo-Ling GL   Walbot Virginia V  

G3 (Bethesda, Md.) 20140617 6


Plants lack a germ line; consequently, during reproduction adult somatic cells within flowers must switch from mitotic proliferation to meiosis. In maize (Zea mays L.) anthers, hypoxic conditions in the developing tassel trigger pre-meiotic competence in the column of pluripotent progenitor cells in the center of anther lobes, and within 24 hr these newly specified germinal cells have patterned their surrounding neighbors to differentiate as the first somatic niche cells. Transcriptomes were ana  ...[more]

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