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RNA sequencing analysis of gene expression in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASC) [Ex.1]

ABSTRACT: We analyzed the whole genome expression profile of NTAPP-exposed ASCs to understand the molecular mechanism of the activated proliferation of adult stem cells by NTAPP exposure.

ORGANISM(S): Homo sapiens

PROVIDER: GSE131384 | GEO | 2020/01/31

REPOSITORIES: GEO

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RNA sequencing analysis of gene expression in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASC) [Ex.2]

Project description:We analyzed the whole genome expression profile of NTAPP-exposed ASCs to understand the molecular mechanism of the activation of adult stem cells proliferation by NTAPP exposure through nitirc oxide response.

2020-01-31 | GSE131385 | GEO

RNA sequencing analysis of the whole genome expression profile in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASCs)

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-01-31 | GSE131386 | GEO

RNA sequencing analysis of gene expression in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASC) [Ex.2]

Project description:RNA sequencing analysis of gene expression in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASC) [Ex.2]

| PRJNA543465 | ENA

RNA sequencing analysis of gene expression in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASC) [Ex.1]

Project description:RNA sequencing analysis of gene expression in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASC) [Ex.1]

| PRJNA543466 | ENA

RNA sequencing analysis of the whole genome expression profile in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASCs)

Project description:RNA sequencing analysis of the whole genome expression profile in non-thermal atmospheric pressure plasma (NTAPP)-exposed and -unexposed human adipose tissue-derived stem cells (ASCs)

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Plant Disease Control by Non-Thermal Atmospheric-Pressure Plasma.

Project description:Disease stresses caused by pathogenic microorganisms are increasing, probably because of global warming. Conventional technologies for plant disease control have often revealed their limitations in efficiency, environmental safety, and economic costs. There is high demand for improvements in efficiency and safety. Non-thermal atmospheric-pressure plasma has demonstrated its potential as an alternative tool for efficient and environmentally safe control of plant pathogenic microorganisms in many studies, which are overviewed in this review. Efficient inactivation of phytopathogenic bacterial and fungal cells by various plasma sources under laboratory conditions has been frequently reported. In addition, plasma-treated water shows antimicrobial activity. Plasma and plasma-treated water exhibit a broad spectrum of efficiency in the decontamination and disinfection of plants, fruits, and seeds, indicating that the outcomes of plasma treatment can be significantly influenced by the microenvironments between plasma and plant tissues, such as the surface structures and properties, antioxidant systems, and surface chemistry of plants. More intense studies are required on the efficiency of decontamination and disinfection and underlying mechanisms. Recently, the induction of plant tolerance or resistance to pathogens by plasma (so-called "plasma vaccination") is emerging as a new area of study, with active research ongoing in this field.

| S-EPMC7034391 | biostudies-literature

Adipose-derived stromal/stem cells (ASC)

Project description:Adipose-derived stromal/stem cells (ASC) capable of multipotential differentiation can be isolated with high yield from human subcutaneous lipoaspirates. This study reports our recent experience isolating and immunophenotypically characterizing ASCs from >60 human subjects

2010-10-01 | GSE18391 | GEO

Atmospheric-Pressure Cold Plasma Induces Transcriptional Changes in Ex Vivo Human Corneas.

Project description:BackgroundAtmospheric pressure cold plasma (APCP) might be considered a novel tool for tissue disinfection in medicine since the active chemical species produced by low plasma doses, generated by ionizing helium gas in air, induces reactive oxygen species (ROS) that kill microorganisms without substantially affecting human cells.ObjectivesIn this study, we evaluated morphological and functional changes in human corneas exposed for 2 minutes (min) to APCP and tested if the antioxidant n-acetyl l-cysteine (NAC) was able to inhibit or prevent damage and cell death.ResultsImmunohistochemistry and western blotting analyses of corneal tissues collected at 6 hours (h) post-APCP treatment demonstrated no morphological tissue changes, but a transient increased expression of OGG1 glycosylase that returned to control levels in 24 h. Transcriptome sequencing and quantitative real time PCR performed on different corneas revealed in the treated corneas many differentially expressed genes: namely, 256 and 304 genes showing expression changes greater than ± 2 folds in the absence and presence of NAC, respectively. At 6 h post-treatment, the most over-expressed gene categories suggested an active or enhanced cell functioning, with only a minority of genes specifically concerning oxidative DNA damage and repair showing slight over-expression values (<2 folds). Moreover, time-related expression analysis of eight genes up-regulated in the APCP-treated corneas overall demonstrated the return to control expression levels after 24 h.ConclusionsThese findings of transient oxidative stress accompanied by wide-range transcriptome adjustments support the further development of APCP as an ocular disinfectant.

| S-EPMC4512711 | biostudies-literature

Non-thermal atmospheric-pressure plasma possible application in wound healing.

Project description:Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

| S-EPMC4256026 | biostudies-other

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways.

Project description:Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.

| S-EPMC5171835 | biostudies-literature

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