Genomics

Dataset Information

0

The Ccr4-Not complex monitors the translating ribosome for codon optimality


ABSTRACT: Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo–electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in Saccharomyces cerevisiae. This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE131408 | GEO | 2020/04/16

REPOSITORIES: GEO

Similar Datasets

2020-04-16 | GSE144250 | GEO
| PRJNA543493 | ENA
| PRJNA603124 | ENA
2015-03-17 | GSE57385 | GEO
2016-05-11 | GSE81269 | GEO
2023-04-02 | GSE171411 | GEO
| PRJNA316313 | ENA
2020-05-05 | GSE133392 | GEO
| E-GEOD-71609 | biostudies-arrayexpress
2022-07-09 | GSE207584 | GEO