Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA-seq of the developing C57/BL6 mouse back skin at E13.5, E16.5 and P0

ABSTRACT: We performed single-cell RNA seq on C57/BL6 mouse back skin at E13.5, E16.5, and P0 to study embryonic hair follicle development. We analyzed 15,086 single cell transcriptome profiles from E13.5, E16.5 and newborn mice (postnatal day 0, P0) dorsal skin cells across hair follicle induction, organogenesis, cytodifferentiation stage. Based on t-distributed Stochastic Neighbor Embedding (tSNE) clustering, we identified 14 cell clusters from skin cells and delineated their cell identity gene expression profile. By using Monocle pseudotime ordering analysis, we constructed epithelium/dermal cell lineage differentiation trajectory and revealed sequential activation of key regulons involved during embryonic hair follicle morphogenesis. Our findings here provide molecular landscape during hair follicle epithelium/dermal cell lineage fate decisions.

ORGANISM(S): Mus musculus

PROVIDER: GSE131498 | GEO | 2020/07/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells

Project description:Global expression analysis of neural crest-like skin-derived precursors (SKPs) and Sox2-positive follicle dermal cells that SKPs originate from. In spite of the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we provide evidence that SKPs, multipotent neural crest-like skin-derived precursors, represent an adult dermal stem cell. When transplanted into adult skin, SKPs can reconstitute the adult dermis, contribute to dermal wound-healing, home to a hair follicle niche, and instruct epidermal cells to make hair follicles. Hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. The endogenous origin of SKPs are Sox2-positive follicle dermal cells that share a similar global gene expression profile with SKPs. These endogenous cells home back to their follicle niche, induce hair follicle morphogenesis, and differentiate into neural and dermal progeny. Hair follicle-associated dermal cells will move out of their niche to contribute to dermal maintenance and wound-healing. These studies therefore identify a dermal stem cell, and provide a biological rationale for the presence of a multipotent precursor within adult dermis, findings with important therapeutic implications.

2009-10-23 | GSE18690 | GEO

A single-cell transcriptome atlas during Cashmere goat hair follicle morphogenesis

Project description:Cashmere, also known as soft gold, is produced from secondary hair follicles in Cashmere goats and it’s therefore of significance to investigate the molecular profiles during Cashmere goat hair follicle development. However, our current understanding of the machinery underlying Cashmere goat hair follicle remains largely unexplored and researches regarding hair follicle development mainly used the mouse as a research model. To provides comprehensively understanding on the cellular heterogeneity and cell lineage cell fate decisions, we performed single-cell RNA sequencing on 19,705 single cells from induction (embryonic day 60), organogenesis (embryonic day 90) and cytodifferentiation (embryonic day 120) stage fetus Cashmere goat dorsal skin. Unsupervised clustering analysis identified 16 cell clusters and their corresponding cell types were also successfully characterized. Based on cell lineage inference, we revealed detailed transcriptional gene expression profiles during dermal and epidermal lineage cell fate decisions. These works together delineate unparalleled molecular profiles of different cell populations during Cashmere goat hair follicle morphogenesis and provide a valuable resource for identifying biomarkers during Cashmere goat hair follicle development.

2020-10-18 | GSE144351 | GEO

Dkk4 and Eda regulate distinctive developmental mechanisms for subtypes of mouse hair-2

Project description:Dkk4 transgenic mice in Tabby background lack hair follicle formation. Gene expression profiling showed significant down regulation of Shh pathway genes at E16.5 and E17.5, the secondary hair follicle induction stages. Keywords: development or differentiation design,genetic modification design To define target genes of Dkk4 during secondary hair follicle induction, we carried out microarray experiments with mouse back skin samples that from 2 developmental time points including E16.5 and E17.5 of Tabby and Tabby background Dkk4 transgenic mice.

2010-09-03 | E-GEOD-19312 | biostudies-arrayexpress

Gene expression profiling of adult hair follicle dermal stem cells and progeny, dermal sheath and dermal papilla cells

Project description:Here we have developed a novel FACS strategy to prospectively isolate hair follicle dermal stem cells, dermal sheath and dermal papilla cells from adult skin initiating synchronous hair follicle regeneration and identified Hic1 as a marker of hfDSCs and Rspondins as stimulators of hfDSCs and epithelial cells, and subsequently hair follicle regeneration

2020-02-03 | GSE109256 | GEO

Dynamic networks of lymphatic vessels interconnect nodes of neighboring hair follicles across the skin

Project description:Dermal lymphatics form a network that connects all the hair follicles in skin and localize in proximity to the Hair Follicle Stem Cell. RNA sequencing analyses of isolated dermal lymphatics at two different time points of the hair follicle cycle (P55 and P70) indicate the existence of dynamic signaling networks associated with lymphatic remodeling, immune trafficking, and HF signaling.

2019-01-01 | GSE102463 | GEO

Identification of transcriptional targets of FGF20.

Project description:Fgf20 is expressed from the hair follicle placode and is required for development of the dermal condensate. Here we used RNAseq to identify the immediate transcriptional targets of FGF20 in Fgf20-/- dermis at E13.5, a timepoint immediately prior to dermal condensate initiation.

2018-08-01 | GSE110459 | GEO

Comparative gene expression analysis of 2 subpopulations of dermal papilla cells.

Project description:Different types of hair follicles can be found in the skin of mice. It is believed that the signals that control hair follicle differentiation arise from cells in a structure called the dermal papilla. Understanding the nature of those signals is of interest for the biology of the normal tissue. We have developed a technique for isolation of dermal cells by enzymatic digestion of intact skin. We have identified two subpopulations of cells that can be separated by FACS. The Sox2-positive CD133-positive cells are found exclusively in the dermal papillae of guard/awl/auchene hairs, while Sox2-negative, CD133-positive cells are found in the other hair follicle types. We compared these populations with unfractionated dermal cells. We isolated the following 3 populations of cells from the back skin of neonatal mice (P2) by Flow Cytometry: 1) GFP-CD133- Total dermal cells 2) GFP-CD133+ Dermal Papilla cells 3) GFP+CD133+ Dermal Papilla cells The yield is approximately 50,000 cells of each population.

2009-06-26 | E-GEOD-16801 | biostudies-arrayexpress

Dkk4 and Eda regulate distinctive developmental mechanisms for subtypes of mouse hair-1

Project description:Dkk4 transgenic mice in wild-type background showed abnormal hair subtype formation. Gene expression profiling showed significant down regulation of hair follicle-related keratin genes and hair keratin-associated protein genes at E18.5 and P1. Keywords: development or differentiation design,genetic modification design To define target genes of Dkk4 during hair follicle development, we carried out microarray experiments with mouse back skin samples that from 4 developmental time points including E14.5, E16.5, E18.5 and P1 of wild-type and wild-type background Dkk4 transgenic mice.

2010-09-03 | E-GEOD-19309 | biostudies-arrayexpress

A transcriptome atlas of embryonic skin

Project description:We co-isolated hair follicle placode and dermal condensate cells along with other specific cell types from E14.5 embryonic mouse skin. With next-generation RNA-sequencing we defined gene expression patterns in the context of the entire embryonic skin. FACS was used to isolate specific cell types from E14.5 embryonic mouse skin.

2015-08-07 | E-GEOD-70288 | biostudies-arrayexpress

Comparative gene expression analysis of 2 subpopulations of dermal papilla cells.

2009-06-26 | GSE16801 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data