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HIV-1 RNA structure and heterogeneity analysis

ABSTRACT: The goal of this set of experiments is to determine the structure of HIV-1 NL4-3 and NHG RNA structure using a novel algorithm that allows for the identification of multiple RNA folding conformations. The input data used for this algorithm is DMS-MaPseq data. We focused on two specific areas of the HIV-1 genome, the Rev Response Element (RRE) and A3 splice acceptor site, as well as probing the whole HIV-1 genome. RNA is DMS-modified in vitro and in vivo as indicated. The goal of this data set was to test the novel alternative RNA structure detecting algorithm (DREEM) using RNA molecules with known structures.

ORGANISM(S): Human immunodeficiency virus 1 synthetic construct Homo sapiens Vibrio vulnificus

PROVIDER: GSE131506 | GEO | 2020/01/14

REPOSITORIES: GEO

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