RNA-RNA interactomes of Hfq and ProQ reveal overlapping and competing roles for RNA chaperones

ABSTRACT: In many bacteria, the base pairing between most small regulatory RNAs (sRNAs) and their targets is facilitated by the Hfq RNA chaperone. However, recent studies have shown FinO-domain proteins also bind sRNAs. To compare the contributions of Hfq and the FinO-domain ProQ protein in Escherichia coli, we carried out RIL-seq, which allows global identification of two RNAs bound to the same protein. We detected hundreds of RNA pairs on ProQ. Intriguingly, 33% of the ProQ-bound RNA pairs are also found associated with Hfq, suggesting overlapping, complementary or competing roles for the two proteins.

ORGANISM(S): Escherichia coli K-12

PROVIDER: GSE131520 | GEO | 2019/12/08

REPOSITORIES: GEO