Transcriptomics

Dataset Information

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Wt1 regulates proliferation and differentiation of neural progenitors during brain development


ABSTRACT: Purpose: To gain a total insight into how Wt1 regulates embryonic neurogenesis, RNA-seq was performed to compare the different expressed genes ar E13 Methods: Total RNA were extracted from E13 telencephalic tissue of Wt1fl/fl and Wt1CKO mice. Then the total RNA was quality controlled and quantified using an Agilent 2100 Bioanalyzer. After converting to cDNA and building library, high-throughput sequencing was performed using the Illumina HiSeq 2500 platform in Annoroad Genomics. Results: Approximately approximately one thousand transcripts showed differential expression between the Wt1fl/fl and Wt1CKO mice brain cortex, with a fold change ≥1.5 and p value <0.05. These results indicated the importance of Wt1 in cortical development. Conclusions:RNA-seq based transcriptome characterization would provide a global understanding how Wt1 gene contribute to brain cortical development.

ORGANISM(S): Mus musculus

PROVIDER: GSE131694 | GEO | 2019/05/24

REPOSITORIES: GEO

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