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A fully defined protocol for differentiation of human pluripotent stem cells into forebrain neural progenitors

ABSTRACT: Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) were differentiated into forebrain neural progenitors using a fully defined protocol with dual SMAD-inhibition. Cells were taken at four time points to monitor temporal changes in the transcriptome over the course of differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE131697 | GEO | 2020/01/14

REPOSITORIES: GEO

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A fully defined protocol for differentiation of human pluripotent stem cells into forebrain neural progenitors

Project description:A fully defined protocol for differentiation of human pluripotent stem cells into forebrain neural progenitors

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VEGF Isoform Transcriptome Changes in the E9.5 Murine Forebrain

Project description:Regulation of neural stem cell (NSC) fate decisions is critical during the transition from a multicellular mammalian forebrain neuroepithelium to the multilayered neocortex. Forebrain development requires coordinated vascular investment alongside NSC differentiation. Vascular endothelial growth factor A (Vegf) has proven to be a pleiotrophic gene whose multiple protein isoforms regulate a broad range of effects in neurovascular systems. To test the hypothesis that the Vegf isoforms (120, 164, and 188) are required for normal forebrain development, we analyzed the forebrain transcriptome of mice expressing specific Vegf isoforms, Vegf120, VegfF188, or a combination of Vegf120/188. Transcriptome analysis identified differentially expressed genes in embryonic day (E) 9.5 forebrain, a time point preceding dramatic neuroepithelial expansion and vascular investment in the telencephalon. Meta-analysis identified gene pathways linked to chromosome-level modifications, cell fate regulation, and neurogenesis that were altered in Vegf isoform mice. Twelve E9.5 wildtype forebrain samples were compared to four E9.5 Vegf120 mouse forebrains, four E9.5 Vegf188 mouse forebrains, three E9.5 Vegf120/188 mouse forebrains, and four E11.5 wild type forebrains using the Mouse 430. 2.0 Affymetrix GeneChip. This study comprises of new samples and reanalysis of Samples from GSE30767 and GSE8091.

2013-11-21 | E-GEOD-52542 | biostudies-arrayexpress

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Project description:RNA-seq samples from 3 species across a differentiation from induced pluripotent stem cells to neural progenitor cells were generated to study gene expression evolution. Briefly, previously generated urinary stem cell derived iPSCs of 3 human (Homo sapiens) individuals (3 clones), 1 gorilla (Gorilla gorilla) individual and fibroblast derived cynomolgus macaque (Macaca fascicularis) iPSCs of 2 individuals (4 clones) (Geuder et al. 2021) were differentiated to neural progenitor cells via dual-SMAD inhibition as three-dimensional aggregation culture (Chambers et al. 2009; Ohnuki et al. 2014). Bulk RNA-seq libraries of iPSCs and NPCs were generated using prime-seq protocol (Janjic et al. 2022).

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Expression of constitutively active FOXO3 in murine forebrain leads to a loss of neural progenitors

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2012-09-13 | GSE21089 | GEO

Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics.

Project description: Not available

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Analysis of ablation of mitochondrial fission in adult forebrain neurons

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2016-08-25 | PXD004890 | Pride

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