Effect of IL-12 on CTL gene expression
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE13173 | GEO | 2009/02/01
SECONDARY ACCESSION(S): PRJNA109645
REPOSITORIES: GEO
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Project description:The goal was to determine how IL-12 affects gene expression by murine CTL. Experiment Overall Design: Following RBC lysis, splenocytes from OT-1 TCR transgenic mice (5 million/ml) were cultured in six well-plates (5-6ml/well) in IMDM supplemented with 10% FCS and 55μM 2-ME with 1μM SIINFEKL. After 4 days, the cells were harvested by purification with Ficoll-Paque PLUS and placed in fresh medium containing 0 or 20ng/ml rmIL-12. Twenty-four hours later the cells were harvested and used in experiments. The live cells harvested were >98% CD8+Vα2+ (OT-1).
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