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Effect of SAICAR accumulation on global transcription

ABSTRACT: We investigated the effects of SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT comparison to determine the effects associated to accumulation of SAICAR alone. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not SAICAR.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE13184 | GEO | 2009/05/15

SECONDARY ACCESSION(S): PRJNA114101

REPOSITORIES: GEO

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Effect of AICAR and SAICAR accumulation on global transcription (first part)

Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives.

2009-05-15 | GSE13182 | GEO

Effect of AICAR and SAICAR accumulation on global transcription (second part)

Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT data set to determine the transcriptional effects associated to AICAR/SAICAR accumulation and those due to ade16 and ade17 deletions. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or lacking AICAR and SAICAR nucleotide derivatives.

2009-05-15 | GSE13183 | GEO

Role of the Pho4p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Pho4p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho4 deletion context.

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Role of the Pho2p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Pho2p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho2 deletion context.

2009-05-15 | GSE13185 | GEO

Role of the Bas1p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Bas1p in the transcriptional response due to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a bas1 deletion context.

2009-05-15 | GSE13187 | GEO

Role of the Snf1p protein kinase in the transcriptional response associated to AICAR and SAICAR accumulation

Project description:We investigated the role of Snf1p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives in a snf1 deletion context.

2009-05-15 | GSE13188 | GEO

Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain. The ade3 mutant strain (Y4143) was compared to the WT strain (Y2946). For each comparison, we made 2 technical replicates, swapping dyes.

2011-08-31 | E-GEOD-29021 | biostudies-arrayexpress

Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain.

2011-09-01 | GSE29021 | GEO

Tye7 regulates yeast Ty1 retrotransposon sense and antisense transcription in response to adenylic nucleotides stress

Project description:We investigated the effects of adenine starvation on the global yeast transcriptome. In this work, global transcription analyses were combined with measurements of intracellular nucleotides pools by HPLC.

2012-02-10 | GSE28579 | GEO

Effect of adk1 knock-out mutation on global transcription

Project description:We investigated the effects of the variation in intracellular adenylic nucleotides pools due to the absence of the adenylate kinase Adk1p on the global yeast transcriptome. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription on total RNA from cells containing or not Adk1p.

2008-04-17 | GSE9553 | GEO

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