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Time course of the Arabidopsis root stem cells

ABSTRACT: The goal of this dataset was to transcriptionally profile the Arabidopsis root stem cells over developmental time. To accomplish this, we protoplasted roots expressing PET111:GFP every 8 hours from 4 days to 6 days old. We then collected GFP negative cells from the PET111:GFP line using cell sorting, as these cells represent the stem cell population. RNA was extracted from cells, libraries were prepared, and RNA-seq was performed. FPKM values were calulcated using Cufflinks.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE131988 | GEO | 2019/10/15

REPOSITORIES: GEO

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