Similar Datasets
Project description:Ribosome TRAP (affinity purification) from Glutamaterigc or GABAergic neurons of the Bed Nucleus Stria Terminalis (BNST) expressing Cre, enabling expression of a floxed TRAP reporter delivered by retrofecting AAV injected into the parabrachial nucleus (PBN) of mice.
2021-03-02 | GSE133484 | GEO
Project description:Both the amygdala and the bed nucleus of the stria terminalis (BNST) have been implicated in maladaptive anxiety characteristic of anxiety disorders. However, the underlying circuit and cellular mechanisms have remained elusive. Here we show that mice with Erbb4 gene deficiency in somatostatin-expressing (SOM+) neurons exhibit heightened anxiety as measured in the elevated plus maze test and the open field test, two assays commonly used to assess anxiety-related behaviors in rodents. Using a combination of electrophysiological, molecular, genetic and pharmacological techniques we demonstrate that the abnormal anxiety in the mutant mice is caused by enhanced excitatory synaptic inputs onto SOM+ neurons in the central amygdala (CeA), and the resulting reduction in inhibition onto downstream SOM+ neurons in the BNST. Notably, our results indicate that an increase in dynorphin signaling in SOM+ CeA neurons mediates the paradoxical reduction in inhibition onto SOM+ BNST neurons, and that the consequent enhanced activity of SOM+ BNST neurons is both necessary for and sufficient to drive the elevated anxiety. Finally, we show that the elevated anxiety and the associated synaptic dysfunctions and increased dynorphin signaling in the CeA-BNST circuit of the Erbb4 mutant mice can be recapitulated by stress in wild-type mice. Together, our results unravel previously unknown circuit and cellular processes in the central extended amygdala that can cause maladaptive anxiety.
2018-05-31 | GSE113979 | GEO
Project description:We used translating ribosome affinity purification (TRAPseq) to profile genetically labeled neurons in the bed nucleus of the stria terminalis (BNST), preoptic area (POA), medial amygdala (MeA) and ventromedial hypothalamus (VMH) from adult male mice, and from adult female mice modeled to be in two distinct stages of the estrus cycle (estrus, sexually receptive, FR; and diestrues, sexually unreceptive, FNR).
2022-01-21 | GSE183092 | GEO
Project description:To identify distinct transcriptional patterns between the major subcortical dopamine targets commonly studied in addiction we studied differences in gene expression between the bed nucleus of the stria terminalis (BNST), nucleus accumbens (NAc), and dorsal striatum (dStr) using microarray analysis. We first tested for differences in expression of genes encoding transcripts for common neurotransmitter systems as well as calcium binding proteins routinely used in neuroanatomical delineation of brain regions. This a priori method revealed differential expression of corticotropin releasing hormone (Crh), the GABA transporter (Slc6a1), and prodynorphin (Pdyn) mRNAs as well as several others between. Using a Gene ontology tool, functional scoring analysis, and Ingenuity Pathway Analysis, we further identified several physiological pathways that were distinct among these brain regions. These two different analysis both identified calcium signaling, G-coupled protein receptor signaling, and adenylate cyclase-related signaling as significantly different among the BNST, NAc, and dStr. The results support other studies suggesting that crucial pathways involved in neurotransmission are distinct among the BNST, NAc, and dStr, and provide insight into the potential use of pharmacological agents that may target region-specific signaling pathways. Further, these studies provide a framework for future mouse-mouse comparisons of transcriptional profiles after behavioral/pharmacological manipulation. Genome-wide microarray was used to detect gene expression. Global functional profile, transcritional networks and canonical pathways were illustrated from the gene expression patterns. Experiment Overall Design: The genome-wide gene expression of mouse brain region bed nucleus of the stria terminalis, nucleus accumbens, and dorsal striatum were analyzed by Affymetrix Mouse430_2 chip. The expression and functional profiles were compared between these 3 tissues and cross-validated from independant data published before.
2007-11-13 | E-GEOD-5763 | biostudies-arrayexpress
Project description:Extended amygdala prepronociceptin neurons encode rapid changes in arousal state
| PRJNA545834 | ENA
Project description:Chronic psychosocial stress is a risk factor for psychiatric disorders, and genetic factors interact in conferring susceptibility. The chronic social defeat stress (CSDS) mouse model allows identifying factors underlying resilience and susceptibility to chronic psychosocial stress. We used RNA-sequencing to identify genes and pathways affected by CSDS in three brain regions: medial prefrontal cortex (mPFC), ventral hippocampus (vHPC), and bed nucleus of the stria terminalis (BNST), of male mice from strains C57BL/6Crl and DBA/2Crl.
2018-07-04 | GSE109315 | GEO
Project description:Analysis of gene expression in the bed nucleus of the stria terminalis/preoptic area and striatum in response to perinatal testosterone exposure. The hypothesis tested was that differences in DNA methylation would be reflected in differences in gene expression. Analysis of gene expression in the bed nucleus of the stria terminalis/preoptic area and striatum in response to perinatal testosterone exposure. The hypothesis tested was that differences in DNA methylation would be reflected in differences in gene expression.
2013-08-29 | E-GEOD-49986 | biostudies-arrayexpress
Project description:Analysis of gene expression in the bed nucleus of the stria terminalis/preoptic area in a mouse model of Klinefelter Syndrome (the Sex Chromosome Trisomy model). The hypothesis tested was that feminization of partner preference was also reflected on a molecular level Analysis of gene expression in the bed nucleus of the stria terminalis/preoptic area in a mouse model of Klinefelter Syndrome (the Sex Chromosome Trisomy model). The hypothesis tested was that feminization of partner preference was also reflected on a molecular level
2013-07-12 | E-GEOD-48783 | biostudies-arrayexpress
Project description:The effect of photoperiod on gene expression was assessed in punch samples of the bed nucleus of the stria terminalis and medial preoptic area. Long day mice were housed in 16L:8D and short day mice were housed in 8L:16D. Experiment Overall Design: For the medial preoptic area, independent samples were collected from long day (n=3) and short day (n=3) mice. For the bed nucleus of the stria terminalis independent samples were collected from long day (n=3) and short day (n=2) mice.
2008-06-14 | E-GEOD-5795 | biostudies-arrayexpress
Project description:Purpose: This study assessed putative sex differences in miRNA expression in the bed nucleus of the stria terminalis (BNST)—a sexually dimorphic brain region implicated in anxiety—of adult male and female rats that had been exposed throughout adolescence to social isolation (SI) stress. Methods: Male and female Sprague-Dawley rats underwent SI during adolescence or remained grouped housed (GH) till adulthood. Small RNA sequencing was performed on tissue extracted from the anterodorsal BNST. Results: SI compared to GH induced more anxiogenic-like effects in females compared to males and sex-dependent changes in miRNA expression in the BNST. Conclusions: The current study shows that housing conditions (either SI or GH) during adolescence regulates miRNA expression in a sex-specific manner in the sexually dimorphic area of the adBNST
2019-09-24 | GSE131289 | GEO